Bioinformatic pipeline for processing iGUIDE and GUIDE-seq samples.
iGUIDE is a pipeline written in snakemake for processing and analyzing double-strand DNA break events. These events may be induced, such as by designer nucleases like Cas9, or spontaneous, as produced through DNA replication or ionizing radiation. A laboratory bench-side protocol accompanies this software pipeline, and can be found https://doi.org/10.1186/s13059-019-1625-3.
To get started, checkout the iGUIDE documentation at iGUIDE.ReadTheDocs.io.
v1.0.0 (August 15th,2019)
- Release of version 1.0.0!!!
- iGUIDE is a computational pipeline that supports the detection of DSBs induced by designer nucleases
- Aligner support for BLAT and BWA currently implemented, let us know if you would like to see others.
- Flexible pipeline processing built on Snakemake, supports a binning system to better distribute workflow for whichever system it is being processed on
- Documentation supporting a Quickstart and User Guide hosted by ReadTheDocs