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Image_analysis_tools

IMC Analysis

Preprocessing

Extract_single_tiffs.py

A small tool to extract correctly named single-channel .tiff files from Steinbock generated multichannel .tiffs. A Steinbock Panel_file needs to be created before.

Option Description Optional
-i, --input Path to the input directory, where multichannel .tiffs are located No
-o, --output Path to the output directory where the output single-channel .tiffs will be stored No
-p, --panel Exact path to the panel file No
-c, --channels "Comma-separated list of channel indices to extract (e.g., '0,1,2')". If not provided, all channels will be extracted yes

extract_single_tiff_ome.py

A small tool to extract correctly named single-channel .tiff files from Steinbock generated multichannel ome.tiffs. This approach does not need a panel file.

Option Description Optional
-i, --input Path to the input directory, where multichannel .tiffs are located No
-o, --output Path to the output directory where the output single-channel .tiffs will be stored No
-n, --channel_names Comma-separated list of channel names to extract (e.g., 'DAPI,GFP,RFP'). If not provided, all channels will be extracted yes