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User manual
input_type <string> [required]: "reads"
reference_genome <string> [required]: Specify the reference genome representative of a microbial species, in FASTA format. Default: "". This parameter is equivalent to --reference in the --help menu.
age_type <int> [required]: 1
intermediate <string> [optional]: Specify a directory for storing intermediate files. Default: "". This parameter is equivalent to --intermediate_dir in the --help menu.
search_report_mode <"-k,int"> [optional]: The upper limit on the number of alignments Bowtie 2 should report in the search of alignments. Default: "-k,5". (The higher the value is set the more computational time required , but more accurate alignment returned.)
bowtie2_threads <int> [optional]: Specify the thread number for bowtie2 to process each sample. Default: [1]
minimum_mapping_quality <int> [optional]: Bases with quality score lower than the specified value will be ignored in reconstructing genome alignment. Default: [30]
minimum_mapping_length <int> [optional]: Aligned reads with length shorter than the specified value will be ignored in reconstructing genome alignment. Default: [30]
maximum_snp_edit_distance <float> [required]: Reads with SNP edit distance greater than the specified value will be ignored in reconstructing genome alignment. Default: [0.03]
nproc <int> [optional]: Specify the number of processors for handling multiple samples in parallel. Default: [1]
minimum_coverage <int> [optional]: A position with a coverage depth lower than the specified value will be ignored in reconstructing genome alignment. Default: [5]
trim_distance <"int:int"> [optional]: The number of nucleotides to trim at two ends of ancient reads. These positions are likely post-mortem damages. Default: "5:5"
dominant_allele_frequency <float> [optional]: A position with the degree of dominant allele lower than the specified value will not be ignored in reconstructing genome alignment. Default: [0.8]
output_trimmed_reads <int> [optional]: Specify 1 if you need to output aligned trimmed reads which are used in reconstructing genome alignment; Specify 0 if you want to skip this feature. Default: [0]
sample <string> [required]:Specify the directory holding ancient metagenomic reads folders. Default: "". This parameter is equivalent to --ancient_metagenomes in the --help menu.
input_type <string> [required]: "reads"
reference_genome <string> [required]: Specify the reference genome representative of a microbial species, in FASTA format. This parameter is equivalent to --reference in the --help menu.
age_type <int> [required]: 2
intermediate <string> [optional]: Specify a directory for storing intermediate files. Default: "". This parameter is equivalent to --intermediate_dir in the --help menu.
search_report_mode <"-k,int"> [required]: The upper limit on the number of alignments Bowtie 2 should report in the search of alignments. Default: "-k,5". (The higher the value is set the more computational time required , but more accurate alignment returned)
bowtie2_threads <int> [optional]: Specify the thread number for bowtie2 processing each sample. Default: [1]
minimum_mapping_quality <int> [optional]: Bases with quality score lower than the specified value will be ignored in reconstructing genome alignment. Default: [30]
minimum_mapping_length <int> [optional]: Aligned reads with length shorter than the specified value will be ignored in reconstructing genome alignment. Default: [30]
maximum_snp_edit_distance <float> [optional]: Reads with SNP edit distance greater than the specified value will be ignored in reconstructing genome alignment. Default: [0.03]
nproc <int> [optional]: Specify the number of processors for handling multiple samples in parallel. Default: [1]
minimum_coverage <int> [optional]: A position with a coverage depth lower than the specified value will be ignored. Default: [5]
dominant_allele_frequency <float> [optional]: A position with the degree of dominant allele lower than the specified value will not be ignored in reconstructing genome alignment. Default: [0.8]
sample <string> [required]: Specify the directory holding modern metagenomic reads folders. Default: "". This parameter is equivalent to --modern_metagenomes in the --help menu.
input_type <string> [required]: "contigs"
reference_genome <string> [required]: Specify the reference genome representative of a microbial species, in FASTA format. This parameter is equivalent to --reference in the --help menu.
intermediate <string> [optional]: Specify a directory for storing intermediate files. Default: "". This parameter is equivalent to --intermediate_dir in the --help menu.
homolog_length <int> [optional]: The minimum length for the aligned part of a contig to be considered as a homolog in order to be used in reconstructing genome alignment. Default: [500]
homolog_identity <float> [optional]:The minimum identity for the aligned part of a contig to be considered as a homolog in order to be used in reconstructing genome alignment. Default: [95.0]
blastn_threads <int> [optional]: Specify the number of threads used for blastn.
sample <string> [required]: Specify the directory holding assembled contigs files. Default: "". This parameter is equivalent to --genome_assemblies in the --help menu.