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ONT Indel calling on polyploid plant #20

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HenrivdGeest opened this issue Dec 22, 2021 · 0 comments
Open

ONT Indel calling on polyploid plant #20

HenrivdGeest opened this issue Dec 22, 2021 · 0 comments

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@HenrivdGeest
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I have ONT data of a plant genome, which can be a tetraploid or diploid genome. we see (igv) multiple INDELS occuring within the length of single reads. However, I do not see any snps in my region of interest. Can nanocaller find indels without any snps present?
I tried it on a 1kb contig, but although for example gatk does report indels, nanocaller does not seem to find any.
I tried making bams with several coverage cuttoffs (50x,75x,100x), but that does not seem so help. I use the singularity version 1.01.
singularity exec --pwd /app /mnt/local_backup/singularity/nanocaller_1.0.1.sif python NanoCaller_WGS.py -bam sample.bam -ref ref.fa --chrom plant_contig000001 -o nanocaller --sequencing ont -mode indels

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