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04-channel.yaml
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Channel ID:
description: this must match the corresponding field in the OME-XML / TIFF header
type: string
significance: required
Channel Name:
description: this must match the corresponding field in the OME-XML / TIFF header
type: string
significance: required
Cycle Number:
description: 'the cycle # in which the co-listed reagent(s) was(were) used'
type: integer
valid-values:
min: 1.0
significance: recommended
'Sub-Cycle #':
description: 'sub-cycle #'
type: integer
valid-values:
min: 1.0
significance: recommended
Target name:
description: short descriptive name (abbreviation) for this target (antigen)
type: string
significance: required
Antibody Name:
description: short descriptive name for this antibody
type: string
significance: required
RRID identifier:
description: Research Resource Identifier
type: rrid
significance: required
Fluorophore:
description: Fluorescent dye label
type: string
significance: required
Clone:
description: Unique clone identifier
type: string
significance: required
Lot:
description: lot number from vendor
type: string
significance: required
Vendor:
description: Vendor name
type: string
significance: required
'Catalog #':
description: catalog number from vendor
type: string
significance: required
Excitation wavelength:
description: center/peak of the excitation spectrum (nm)
type: float
significance: recommended
Emission wavelength:
description: center/peak of the emission spectrum (nm)
type: float
significance: recommended
Excitation bandwidth:
description: nominal width of excitation spectrum (nm)
type: float
significance: recommended
Emission bandwidth:
description: nominal width of emission spectrum (nm)
type: float
significance: recommended
'Metal isotope: Element':
description: Element abbreviation
type: string
significance: required
'Metal isotope: Mass':
description: Element mass number
type: integer
significance: required
'Oligo barcode: upper strand':
description: DNA barcode used for labeling
type: string
significance: required
'Oligo barcode: lower strand':
description: DNA barcode used for labeling
type: string
significance: required
Dilution:
description: Final dilution ratio used in experiment
type: string
significance: recommended
Concentration:
description: Final concentration used in experiment
type: string
significance: recommended
Passed QC:
description: Identify stains that did not pass QC but are included in the dataset.
type: boolean
significance: recommended
QC details:
description: Comment on why QC failed
type: string
significance: recommended
Comment:
description: Free text field to use if necessary
type: string
significance: recommended