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lumpy returns more than expected variants #382

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anoronh4 opened this issue Jul 1, 2022 · 0 comments
Open

lumpy returns more than expected variants #382

anoronh4 opened this issue Jul 1, 2022 · 0 comments

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@anoronh4
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anoronh4 commented Jul 1, 2022

i have run lumpy express as follows:

singularity exec \
	-e --no-home \
	-B /path/to/bams/ \
	../lumpy_v0.2.13-2d611fa.sif \
	lumpyexpress \
	  -B ${bamTumor},${bamNormal} \
	  -S ${idTumor}.splitters.bam,${idNormal}.splitters.bam \
          -D ${idTumor}.discordants.bam,${idNormal}.discordants.bam \
	  -o ${idTumor}__${idNormal}.vcf

I am finding that in our test, lumpy generates a lot more passing variants compared to other callers, for one sample we have less than 200 variants in each of 3 other callers, and lumpy produces >10000. just wondering if there was an acceptable way to filter the calls, particularly for tumor/normal pairs where we are trying to find variants specific to the tumor.

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