We suggest 5Gb memory and 16 cpu for mecat alignment.
The size of memory depends on the file size of the fastq. Usually 15Gb fastq input requires 20Gb for blasr alignment. Since run_pbjelly have filtered most of reads, ~80%, we recommend 2Gb memory and 20 cpu for blasr alignment and 16 cpu for the local assembly in PBJelly.
You can easily change these settings in the config.cfg file.
For a genome ~600Mb size with 100x PacBio, the storage for each folder is:
To save you storage, you can nohup the shell 'nohup_rm.sh' after you finished runpbjelly.