From 86ef8155a7cbc9cf30260fbfec9d94de80cc1b6e Mon Sep 17 00:00:00 2001 From: Anh Nguyet Vu Date: Wed, 22 May 2024 02:10:22 -0600 Subject: [PATCH 1/6] Update data and meta --- public/nst_nfosi_ntap/data_clinical_patient.txt | 4 ++-- public/nst_nfosi_ntap/data_clinical_sample.txt | 4 ++-- public/nst_nfosi_ntap/data_expression.txt | 3 +++ public/nst_nfosi_ntap/data_expression_raw.txt | 3 +++ public/nst_nfosi_ntap/data_mutations.txt | 4 ++-- public/nst_nfosi_ntap/meta_mutations.txt | 2 +- 6 files changed, 13 insertions(+), 7 deletions(-) create mode 100644 public/nst_nfosi_ntap/data_expression.txt create mode 100644 public/nst_nfosi_ntap/data_expression_raw.txt diff --git a/public/nst_nfosi_ntap/data_clinical_patient.txt b/public/nst_nfosi_ntap/data_clinical_patient.txt index 5d27629cd6..9c54cb3bc0 100644 --- a/public/nst_nfosi_ntap/data_clinical_patient.txt +++ b/public/nst_nfosi_ntap/data_clinical_patient.txt @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:cc8416ea6960a03a44d585469037f25ae65358d273efa9f1d805228a774f85ef -size 621 +oid sha256:9efae1b1162da1b938a698801e6642bbec01c9d3c5d6a1b55af79e0d222c04e5 +size 882 diff --git a/public/nst_nfosi_ntap/data_clinical_sample.txt b/public/nst_nfosi_ntap/data_clinical_sample.txt index aada593fc2..88391e587d 100644 --- a/public/nst_nfosi_ntap/data_clinical_sample.txt +++ b/public/nst_nfosi_ntap/data_clinical_sample.txt @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:c6e650166ab96237c6b00d6b4fb5ea90462c3573df9a837736b84aedb00871e4 -size 2465 +oid sha256:d4b9c0e20d792646bb8d292fa664c8210614b208845e67eab6d0f7323d7a043d +size 9753 diff --git a/public/nst_nfosi_ntap/data_expression.txt b/public/nst_nfosi_ntap/data_expression.txt new file mode 100644 index 0000000000..aa063c97ec --- /dev/null +++ b/public/nst_nfosi_ntap/data_expression.txt @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:66cf8c6db2f69913a88d90fb69bcdb80caca61929ab49bf9523f99a25e89349d +size 18168712 diff --git a/public/nst_nfosi_ntap/data_expression_raw.txt b/public/nst_nfosi_ntap/data_expression_raw.txt new file mode 100644 index 0000000000..8e373906f4 --- /dev/null +++ b/public/nst_nfosi_ntap/data_expression_raw.txt @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:f182f10d4ee602bd641d206064c307d61b542f757e16a0867c587e6579bb774b +size 11261706 diff --git a/public/nst_nfosi_ntap/data_mutations.txt b/public/nst_nfosi_ntap/data_mutations.txt index 508f20c298..700e140659 100644 --- a/public/nst_nfosi_ntap/data_mutations.txt +++ b/public/nst_nfosi_ntap/data_mutations.txt @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:28069575a6a430b3993f5aaf5b63d3331b2a724bf8f576a492cc017cebe5732b -size 727805 +oid sha256:6edae07377a546f94ae3ce5648d3e658fe5465897e8dd038fcf133769f33858f +size 27722524 diff --git a/public/nst_nfosi_ntap/meta_mutations.txt b/public/nst_nfosi_ntap/meta_mutations.txt index 1aaa645ddd..e568d4cf7c 100644 --- a/public/nst_nfosi_ntap/meta_mutations.txt +++ b/public/nst_nfosi_ntap/meta_mutations.txt @@ -4,5 +4,5 @@ datatype: MAF stable_id: mutations show_profile_in_analysis_tab: true profile_name: Mutations -profile_description: Mutation data from NF-OSI processing. The data were processed using the "sarek" nf-core pipeline. +profile_description: Mutation data from NF-OSI processing. data_filename: data_mutations.txt From 2564b2ce3589cea5825c89b757b93aeb9bb2f2a1 Mon Sep 17 00:00:00 2001 From: Anh Nguyet Vu Date: Wed, 22 May 2024 02:10:43 -0600 Subject: [PATCH 2/6] Regenerate case list --- public/nst_nfosi_ntap/case_lists/cases_all.txt | 4 ++-- public/nst_nfosi_ntap/case_lists/cases_sequenced.txt | 4 ++-- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/public/nst_nfosi_ntap/case_lists/cases_all.txt b/public/nst_nfosi_ntap/case_lists/cases_all.txt index 363a1d2410..8b73f068f7 100644 --- a/public/nst_nfosi_ntap/case_lists/cases_all.txt +++ b/public/nst_nfosi_ntap/case_lists/cases_all.txt @@ -1,6 +1,6 @@ cancer_study_identifier: nst_nfosi_ntap stable_id: nst_nfosi_ntap_all case_list_name: All samples -case_list_description: All samples (19 samples) +case_list_description: All samples (134 samples) case_list_category: all_cases_in_study -case_list_ids: 2-010_Neurofibroma 2-026_Neurofibroma 2-013_Plexiform_Neurofibroma 2-004_Plexiform_Neurofibroma 2-012_Neurofibroma 2-031_Cell_Line 2-017_Neurofibroma 2-021_Neurofibroma 2-019_Neurofibroma 2-029_Neurofibroma 2-023_Plexiform_Neurofibroma 2-014_Neurofibroma 2-001_Plexiform_Neurofibroma 2-006_Plexiform_Neurofibroma 2-032_Plexiform_Neurofibroma 2-015_Plexiform_Neurofibroma 2-031_Malignant_Peripheral_Nerve_Sheath_Tumor 2-016_Neurofibroma 2-015_Neurofibroma +case_list_ids: JH-2-075-3HE68-A JH-2-082-G8357-A JH-2-007-B14BB-AG2A6 JH-2-054-241HF-A JH-2-002-GAF53-A1011 JH-2-085-F2983-F783D JH-2-068-2BHHG-5F9FC JH-2-111-G645D-6C5FF JH-2-009-518B9-A JH-2-016-6H516-A JH-2-010-H915D-A JH-2-017-9FH8F-A JH-2-077-4H749-8GCFH JH-2-014-AH466-96B78 JH-2-055-9F5F3-6G883 JH-2-068-A733G-A JH-2-023-91HBD-A JH-2-001-8A1B1-7B15A JH-2-093-518GA-A JH-2-100-19H33-94G2C JH-2-003-H3G1H-HC8GG JH-2-091-CAA8H-A JH-2-021-6GFE7-A JH-2-068-FH52C-A JH-2-009-2578C-A JH-2-079-CB92G-A JH-2-021-6GFE7-7HBG6 JH-2-002-94FAA-A JH-2-001-8A1B1-A JH-2-095-4H3FE-A JH-2-060-4CE8D-A JH-2-076-41G1H-A JH-2-079-9D31F-A JH-2-006-D6BA4-BAEBD JH-2-092-B52E5-A JH-2-045-GH3AA-A JH-2-045-F7H3C-A JH-2-032-AE88A-A JH-2-031-9GEA9-A JH-2-055-1419H-9GG15 JH-2-002-GAF53-FB9H7 JH-2-029-7AE7F-GC2A3 JH-2-002-GAF53-A71AA JH-2-012-HE58H-A JH-2-013-7886G-A JH-2-017-9FH8F-93DE8 JH-2-029-7AE7F-A JH-2-032-AE88A-G36A1 JH-2-094-5HA24-A JH-2-016-4H597-A JH-2-045-GH3AA-D2C35 JH-2-103-H731E-A JH-2-094-151G9-A JH-2-044-83F15-A JH-2-060-9G31E-HD38A JH-2-002-94FAA-19DGB JH-2-015-55B8D-2B9EB JH-2-096-5E593-A JH-2-091-CAA8H-GDFG1 JH-2-075-4F211-A JH-2-055-FC9GH-A JH-2-090-19D3F-A JH-2-068-A7488-A JH-2-089-H8EBA-HH51C JH-2-002-FF824-A JH-2-092-B4F11-A JH-2-074-HE12C-A JH-2-072-3DD4G-A JH-2-009-518B9-77BH3 JH-2-100-19H33-A JH-2-082-B5148-A92CA JH-2-002-GAF53-2E57B JH-2-023-1G2BC-A JH-2-015-C436G-A JH-2-016-4H597-15C1D JH-2-019-DB5EH-A JH-2-057-37D5D-A JH-2-060-19EC4-A JH-2-004-9D9D7-7EC36 JH-2-023-91HBD-2G2E2 JH-2-104-51E2H-A JH-2-093-214AE-A JH-2-019-DB5EH-C461C JH-2-023-876HC-A JH-2-085-F2983-A JH-2-092-B4F11-2H1C1 JH-2-031-9GEA9-78GAB JH-2-086-5EA11-A JH-2-055-E1381-A JH-2-002-FF824-C5E25 JH-2-054-9255F-A JH-2-089-H8EBA-A JH-2-099-26HCE-A JH-2-002-354A1-A JH-2-023-876HC-622H1 JH-2-104-51E2H-1G93A JH-2-013-7886G-AA816 JH-2-094-5HA24-CC3H4 JH-2-060-99B2D-GG71G JH-2-044-83F15-GA258 JH-2-015-G41AC-A JH-2-086-5EA11-494GE JH-2-023-89E1F-A JH-2-016-6H516-818HB JH-2-111-G645D-A JH-2-010-H915D-BB99B JH-2-076-41G1H-7FGE5 JH-2-026-9FD64-A JH-2-093-214AE-H5A8H JH-2-002-GAF53-3BAGC JH-2-082-G8357-EG55A JH-2-015-6AD96-A JH-2-031-BFF3F-DG6C8 JH-2-014-AH466-A JH-2-076-811H2-A JH-2-015-55B8D-A JH-2-068-2BHHG-A JH-2-061-1H66H-AB35D JH-2-090-19D3F-G36F6 JH-2-012-HE58H-37B26 JH-2-026-9FD64-HF616 JH-2-082-B5148-A JH-2-006-D6BA4-A JH-2-107-AGD39-1CH62 JH-2-075-3HE68-C61FE JH-2-023-9DA42-A JH-2-092-B52E5-BFCBA JH-2-004-9D9D7-A JH-2-090-59DE8-A JH-2-077-88G11-A JH-2-113-7857D-A JH-2-102-3B72A-A JH-2-023-1G2BC-82G48 JH-2-061-1H66H-A diff --git a/public/nst_nfosi_ntap/case_lists/cases_sequenced.txt b/public/nst_nfosi_ntap/case_lists/cases_sequenced.txt index 3c6255a2c0..203a74f844 100644 --- a/public/nst_nfosi_ntap/case_lists/cases_sequenced.txt +++ b/public/nst_nfosi_ntap/case_lists/cases_sequenced.txt @@ -1,6 +1,6 @@ cancer_study_identifier: nst_nfosi_ntap stable_id: nst_nfosi_ntap_sequenced case_list_name: Samples with mutation data -case_list_description: Samples with mutation data (19 samples) +case_list_description: Samples with mutation data (80 samples) case_list_category: all_cases_with_mutation_data -case_list_ids: 2-010_Neurofibroma 2-026_Neurofibroma 2-013_Plexiform_Neurofibroma 2-014_Neurofibroma 2-001_Plexiform_Neurofibroma 2-006_Plexiform_Neurofibroma 2-004_Plexiform_Neurofibroma 2-032_Plexiform_Neurofibroma 2-015_Plexiform_Neurofibroma 2-016_Neurofibroma 2-012_Neurofibroma 2-031_Malignant_Peripheral_Nerve_Sheath_Tumor 2-031_Cell_Line 2-017_Neurofibroma 2-015_Neurofibroma 2-021_Neurofibroma 2-019_Neurofibroma 2-029_Neurofibroma 2-023_Plexiform_Neurofibroma +case_list_ids: JH-2-100-19H33-A JH-2-075-3HE68-A JH-2-082-G8357-A JH-2-113-7857D-A JH-2-015-C436G-A JH-2-054-241HF-A JH-2-002-GAF53-A1011 JH-2-001-8A1B1-A JH-2-009-2578C-A JH-2-057-37D5D-A JH-2-060-19EC4-A JH-2-016-6H516-A JH-2-023-1G2BC-A JH-2-017-9FH8F-A JH-2-068-A733G-A JH-2-023-91HBD-A JH-2-093-214AE-A JH-2-103-H731E-A JH-2-023-876HC-A JH-2-085-F2983-A JH-2-093-518GA-A JH-2-086-5EA11-A JH-2-055-E1381-A JH-2-054-9255F-A JH-2-089-H8EBA-A JH-2-021-6GFE7-A JH-2-068-FH52C-A JH-2-002-354A1-A JH-2-079-CB92G-A JH-2-009-518B9-A JH-2-002-94FAA-A JH-2-045-F7H3C-A JH-2-095-4H3FE-A JH-2-060-4CE8D-A JH-2-076-41G1H-A JH-2-079-9D31F-A JH-2-092-B52E5-A JH-2-045-GH3AA-A JH-2-074-HE12C-A JH-2-032-AE88A-A JH-2-031-9GEA9-A JH-2-023-89E1F-A JH-2-055-1419H-9GG15 JH-2-099-26HCE-A JH-2-002-GAF53-FB9H7 JH-2-111-G645D-A JH-2-012-HE58H-A JH-2-013-7886G-A JH-2-026-9FD64-A JH-2-029-7AE7F-A JH-2-015-6AD96-A JH-2-094-5HA24-A JH-2-016-4H597-A JH-2-014-AH466-A JH-2-076-811H2-A JH-2-015-55B8D-A JH-2-010-H915D-A JH-2-094-151G9-A JH-2-044-83F15-A JH-2-096-5E593-A JH-2-006-D6BA4-A JH-2-104-51E2H-A JH-2-019-DB5EH-A JH-2-023-9DA42-A JH-2-075-4F211-A JH-2-082-B5148-A JH-2-055-FC9GH-A JH-2-090-19D3F-A JH-2-004-9D9D7-A JH-2-090-59DE8-A JH-2-077-88G11-A JH-2-015-G41AC-A JH-2-102-3B72A-A JH-2-002-FF824-A JH-2-092-B4F11-A JH-2-068-A7488-A JH-2-068-2BHHG-A JH-2-091-CAA8H-A JH-2-072-3DD4G-A JH-2-061-1H66H-A From 146dd2f30180f1c05f4a1277c5b5febf046086c6 Mon Sep 17 00:00:00 2001 From: Anh Nguyet Vu Date: Wed, 22 May 2024 09:51:15 -0600 Subject: [PATCH 3/6] Rename and update meta --- .../{data_expression.txt => data_expression_tpm.txt} | 0 public/nst_nfosi_ntap/meta_expression_raw.txt | 8 ++++++++ public/nst_nfosi_ntap/meta_expression_tpm.txt | 8 ++++++++ public/nst_nfosi_ntap/meta_mutations.txt | 2 +- 4 files changed, 17 insertions(+), 1 deletion(-) rename public/nst_nfosi_ntap/{data_expression.txt => data_expression_tpm.txt} (100%) create mode 100644 public/nst_nfosi_ntap/meta_expression_raw.txt create mode 100644 public/nst_nfosi_ntap/meta_expression_tpm.txt diff --git a/public/nst_nfosi_ntap/data_expression.txt b/public/nst_nfosi_ntap/data_expression_tpm.txt similarity index 100% rename from public/nst_nfosi_ntap/data_expression.txt rename to public/nst_nfosi_ntap/data_expression_tpm.txt diff --git a/public/nst_nfosi_ntap/meta_expression_raw.txt b/public/nst_nfosi_ntap/meta_expression_raw.txt new file mode 100644 index 0000000000..f1f3511ea2 --- /dev/null +++ b/public/nst_nfosi_ntap/meta_expression_raw.txt @@ -0,0 +1,8 @@ +cancer_study_identifier: nst_nfosi_ntap +genetic_alteration_type: MRNA_EXPRESSION +datatype: CONTINUOUS +stable_id: rna_seq_mrna +show_profile_in_analysis_tab: true +profile_name: mRNA expression +profile_description: Expression levels +data_filename: data_expression_raw.txt diff --git a/public/nst_nfosi_ntap/meta_expression_tpm.txt b/public/nst_nfosi_ntap/meta_expression_tpm.txt new file mode 100644 index 0000000000..22ee3de8cf --- /dev/null +++ b/public/nst_nfosi_ntap/meta_expression_tpm.txt @@ -0,0 +1,8 @@ +cancer_study_identifier: nst_nfosi_ntap +genetic_alteration_type: MRNA_EXPRESSION +datatype: CONTINUOUS +stable_id: mrna_seq_tpm +show_profile_in_analysis_tab: true +profile_name: Normalized gene expression +profile_description: Normalized gene expression levels (TPM) +data_filename: data_expression_tpm.txt diff --git a/public/nst_nfosi_ntap/meta_mutations.txt b/public/nst_nfosi_ntap/meta_mutations.txt index e568d4cf7c..1aaa645ddd 100644 --- a/public/nst_nfosi_ntap/meta_mutations.txt +++ b/public/nst_nfosi_ntap/meta_mutations.txt @@ -4,5 +4,5 @@ datatype: MAF stable_id: mutations show_profile_in_analysis_tab: true profile_name: Mutations -profile_description: Mutation data from NF-OSI processing. +profile_description: Mutation data from NF-OSI processing. The data were processed using the "sarek" nf-core pipeline. data_filename: data_mutations.txt From 334d2d98fd0645f00370f0e74d7fc5433e83d1dc Mon Sep 17 00:00:00 2001 From: Anh Nguyet Vu Date: Wed, 5 Jun 2024 18:52:04 -0600 Subject: [PATCH 4/6] Add README --- public/nst_nfosi_ntap/README.md | 7 +++++++ 1 file changed, 7 insertions(+) create mode 100644 public/nst_nfosi_ntap/README.md diff --git a/public/nst_nfosi_ntap/README.md b/public/nst_nfosi_ntap/README.md new file mode 100644 index 0000000000..3222b148f3 --- /dev/null +++ b/public/nst_nfosi_ntap/README.md @@ -0,0 +1,7 @@ +## Notes + +The patient cohort is from [The Johns Hopkins NF1 Biospecimen Repository](https://www.synapse.org/Synapse:syn53140231), which is an active biobanking project with rolling releases. It is funded by the [Neurofibromatosis Therapeutic Acceleration Program (NTAP)](https://www.n-tap.org/), led by Christine Pratilas, M.D., and David Loeb, M.D., Ph.D., with contributions from Angela Hirbe, PhD., and additional collaboration, data processing and data management by [NF-OSI / Sage Bionetworks](https://help.nf.synapse.org/NFdocs/about). + +Patient raw sequencing data are generated by the institutional cores and shared on the secure Synapse platform with access restrictions. Sage Bionetworks does additional processing through the funded initiative described here: https://help.nf.synapse.org/NFdocs/data-processing. The [nf-core/rnaseq](https://nf-co.re/rnaseq/) workflow is used for gene expression from bulk RNA-seq and [nf-co.re/sarek](https://nf-co.re/sarek/) is used for somatic variant calling from whole exome sequencing (also for germline variant calling, but this is not shared on cBioPortal and only available on Synapse with access restrictons). After variant calling, variant annotation is done with [vcf2maf](https://github.com/Sage-Bionetworks-Workflows/nf-vcf2maf). + +Sage Bionetworks then appropriately packages the processed gene expression and mutations data with approved clinical data for contribution to cBioPortal, also funded by NTAP. Full clinical data are not contributed due to IRB restrictions. Released patient samples are confirmed with Christine Pratilas through an internal process. Updates may be occasionally made. For questions about clinical data and collaboration, please use the most up-to-date PI contact instructions on the [project space](https://www.synapse.org/Synapse:syn53133024/). From ea5ffb98b6ab5e11918816c4fefb1ad6412328c1 Mon Sep 17 00:00:00 2001 From: Anh Nguyet Vu Date: Wed, 5 Jun 2024 19:08:08 -0600 Subject: [PATCH 5/6] Update gx files, esp. naming --- public/nst_nfosi_ntap/data_expression_raw.txt | 3 --- .../{data_expression_tpm.txt => data_mrnaseq_tpm.txt} | 0 public/nst_nfosi_ntap/meta_expression_raw.txt | 8 -------- .../{meta_expression_tpm.txt => meta_mrnaseq_tpm.txt} | 2 +- 4 files changed, 1 insertion(+), 12 deletions(-) delete mode 100644 public/nst_nfosi_ntap/data_expression_raw.txt rename public/nst_nfosi_ntap/{data_expression_tpm.txt => data_mrnaseq_tpm.txt} (100%) delete mode 100644 public/nst_nfosi_ntap/meta_expression_raw.txt rename public/nst_nfosi_ntap/{meta_expression_tpm.txt => meta_mrnaseq_tpm.txt} (87%) diff --git a/public/nst_nfosi_ntap/data_expression_raw.txt b/public/nst_nfosi_ntap/data_expression_raw.txt deleted file mode 100644 index 8e373906f4..0000000000 --- a/public/nst_nfosi_ntap/data_expression_raw.txt +++ /dev/null @@ -1,3 +0,0 @@ -version https://git-lfs.github.com/spec/v1 -oid sha256:f182f10d4ee602bd641d206064c307d61b542f757e16a0867c587e6579bb774b -size 11261706 diff --git a/public/nst_nfosi_ntap/data_expression_tpm.txt b/public/nst_nfosi_ntap/data_mrnaseq_tpm.txt similarity index 100% rename from public/nst_nfosi_ntap/data_expression_tpm.txt rename to public/nst_nfosi_ntap/data_mrnaseq_tpm.txt diff --git a/public/nst_nfosi_ntap/meta_expression_raw.txt b/public/nst_nfosi_ntap/meta_expression_raw.txt deleted file mode 100644 index f1f3511ea2..0000000000 --- a/public/nst_nfosi_ntap/meta_expression_raw.txt +++ /dev/null @@ -1,8 +0,0 @@ -cancer_study_identifier: nst_nfosi_ntap -genetic_alteration_type: MRNA_EXPRESSION -datatype: CONTINUOUS -stable_id: rna_seq_mrna -show_profile_in_analysis_tab: true -profile_name: mRNA expression -profile_description: Expression levels -data_filename: data_expression_raw.txt diff --git a/public/nst_nfosi_ntap/meta_expression_tpm.txt b/public/nst_nfosi_ntap/meta_mrnaseq_tpm.txt similarity index 87% rename from public/nst_nfosi_ntap/meta_expression_tpm.txt rename to public/nst_nfosi_ntap/meta_mrnaseq_tpm.txt index 22ee3de8cf..a0fe1a0ebb 100644 --- a/public/nst_nfosi_ntap/meta_expression_tpm.txt +++ b/public/nst_nfosi_ntap/meta_mrnaseq_tpm.txt @@ -5,4 +5,4 @@ stable_id: mrna_seq_tpm show_profile_in_analysis_tab: true profile_name: Normalized gene expression profile_description: Normalized gene expression levels (TPM) -data_filename: data_expression_tpm.txt +data_filename: data_mrnaseq_tpm.txt From 3cdb580e80caa1fe189f348990c78b1d6e6b7675 Mon Sep 17 00:00:00 2001 From: Anh Nguyet Vu Date: Wed, 12 Jun 2024 18:55:26 -0600 Subject: [PATCH 6/6] Add somatic status --- public/nst_nfosi_ntap/data_clinical_sample.txt | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/public/nst_nfosi_ntap/data_clinical_sample.txt b/public/nst_nfosi_ntap/data_clinical_sample.txt index 88391e587d..4fdc97e259 100644 --- a/public/nst_nfosi_ntap/data_clinical_sample.txt +++ b/public/nst_nfosi_ntap/data_clinical_sample.txt @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:d4b9c0e20d792646bb8d292fa664c8210614b208845e67eab6d0f7323d7a043d -size 9753 +oid sha256:f0f26d158f64fc035c07bf905677d87c582024468b16e7fb3b2377d0d8548fc4 +size 11521