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rsem-calculate-expression #197

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km-2021 opened this issue May 17, 2023 · 0 comments
Open

rsem-calculate-expression #197

km-2021 opened this issue May 17, 2023 · 0 comments

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@km-2021
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km-2021 commented May 17, 2023

Hi,

I run the rsem-calculate-expression, but fails with the following command:

rsem-parse-alignments ref/human_refseq hoge.temp/hoge hoge.stat/hoge hoge.temp/hoge.bam 3 -tag XM
The SAM/BAM file declares more reference sequences (130527) than RSEM knows (66801)!
"rsem-parse-alignments ref/human_refseq hoge.temp/hoge hoge.stat/hoge.temp/oge.bam 3 -tag XM" failed! Plase check if you provide correct parameters/options for the pipeline!

I would appreciate your guidance on how to deal with this problem.

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