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how to simulate single-end reads? #200

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yhg926 opened this issue Jun 9, 2023 · 0 comments
Open

how to simulate single-end reads? #200

yhg926 opened this issue Jun 9, 2023 · 0 comments

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@yhg926
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yhg926 commented Jun 9, 2023

I want to simulate single-end RNAseq reads that is error-free , uniform distributed; here is my out.model parameter:
0

1

100 101 1
1

0
99 100 1
1

0

1
0

=====
but it reported error when running rem-sim: rsem-simulate-reads syn_4exons.rsem ./out.model sample4.out.isoforms.results 0 1000000 syn_4exons.sim
Refs.loadRefs finished!
rsem-simulate-reads: RSPD.h:154: void RSPD::read(FILE*): Assertion `fscanf(fi, "%lf", &pdf[i]) == 1' failed.
Aborted (core dumped)

so how can i configure the model file for this purpose?
Many thanks.

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