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Error: Failed to open output file #5

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mforthman opened this issue Aug 28, 2019 · 0 comments
Open

Error: Failed to open output file #5

mforthman opened this issue Aug 28, 2019 · 0 comments

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@mforthman
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I am using a script that has worked very well in the past (error correcting sequence reads for ~200 samples). I'm now trying to process about 90 samples and it failed for every one, with the same kind of error:

Error: Failed to open output file 'quorum_output/Acidomeria-cinctipes-CMF-0941_ALL_READS_mer_database.jf'.
Usage: create_database_cmdline [options] reads:path+
Use --help for more information
Creating the mer database failed. Most likely the size passed to the -s switch is too small. at /apps/masurca/2.3.2/bin/quorum line 143.
Wed Aug 28 07:40:12 EDT 2019

I've always used the default -s value. Given the error, I've increased this to 50M, 100M, 1000M, and 10000M, and I continuously get the same error across all sample. I'm starting to suspect the cause may be something other than the -s, but I'm not sure what this could be.

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