diff --git a/src/codecs/avif/yuv.rs b/src/codecs/avif/yuv.rs
index a41b99a7a3..747cce8381 100644
--- a/src/codecs/avif/yuv.rs
+++ b/src/codecs/avif/yuv.rs
@@ -350,6 +350,11 @@ where
         },
         "Unsupported bit depth and data type combination"
     );
+    assert_ne!(
+        matrix,
+        YuvStandardMatrix::Identity,
+        "Identity matrix cannot be used on 4:0:0"
+    );
 
     let y_plane = image.y_plane;
     let y_stride = image.y_stride;
@@ -527,6 +532,11 @@ where
         },
         "Unsupported bit depth and data type combination"
     );
+    assert_ne!(
+        matrix,
+        YuvStandardMatrix::Identity,
+        "Identity matrix cannot be used on 4:2:0"
+    );
     let y_plane = image.y_plane;
     let u_plane = image.u_plane;
     let v_plane = image.v_plane;
@@ -903,6 +913,11 @@ where
         },
         "Unsupported bit depth and data type combination"
     );
+    assert_ne!(
+        matrix,
+        YuvStandardMatrix::Identity,
+        "Identity matrix cannot be used on 4:2:2"
+    );
     let y_plane = image.y_plane;
     let u_plane = image.u_plane;
     let v_plane = image.v_plane;