BlueLight.bsh

import org.micromanager.api.AcquisitionOptions;
import java.lang.System;

gui.closeAllAcquisitions();
//gui.clearMessageWindow();



numBefore = 2;
numAfter = 5;
interval = 500;
exposure = 2000;
gain = 500;
gainon = "On";
acqname = "29-05-12";

YFPchan = "Direct Digital Out 2";
bluechan = "Direct Digital Out 1";


gui.openAcquisition(acqname,"C:/Users/User/Desktop", numBefore + numAfter, 1, 1 );

// set up the camera
cameraName = mmc.getCameraDevice();
mmc.setExposure(exposure);
mmc.setProperty( cameraName, "Binning", "1");
mmc.setProperty( cameraName, "EMSwitch", gainon );
mmc.setProperty( cameraName, "Gain", gain );

// turn off the LEDs
mmc.setProperty( "OptoLED", "Direct Digital Out 0", "0" );
mmc.setProperty( "OptoLED", "Direct Digital Out 3", "0" );

mmc.setProperty( "OptoLED", YFPchan, "0" );
mmc.setProperty( "OptoLED", bluechan, "0" );

// open the shutter
mmc.setProperty( "Core", "AutoShutter", "0" );
mmc.setProperty( "OptoLED", "Shutter", "0" );

mmc.setProperty( "OptoLED", YFPchan, "0" );
mmc.setProperty( "OptoLED", bluechan, "0" );

for (int i=0; i<numBefore; i++)	{
	now = System.currentTimeMillis();
	mmc.setProperty( "OptoLED", YFPchan, "1" );
	gui.snapAndAddImage(acqname, i, 0, 0 );
	mmc.setProperty( "OptoLED", YFPchan, "0" );
	itTook = System.currentTimeMillis() - now;
	if ( itTook < interval )
		gui.sleep( interval - itTook );
}

mmc.setProperty( "OptoLED", bluechan, "1" );
for (int i=0; i<numAfter; i++)	{
	now = System.currentTimeMillis();
	mmc.setProperty( "OptoLED", YFPchan, "1" );
	gui.snapAndAddImage(acqname, numBefore + i, 0, 0 );
	mmc.setProperty( "OptoLED", YFPchan, "0" );
	itTook = System.currentTimeMillis() - now;
	if ( itTook < interval )
		gui.sleep( interval - itTook );
}
mmc.setProperty( "OptoLED", bluechan, "0" );


gui.refreshGUI();