diff --git a/gui/locales/messages.pot b/gui/locales/messages.pot
index 517423d42078..4d588a5a9eda 100644
--- a/gui/locales/messages.pot
+++ b/gui/locales/messages.pot
@@ -1600,6 +1600,10 @@ msgctxt "split-tunneling-view"
 msgid "Please try again or send a problem report."
 msgstr ""
 
+msgctxt "split-tunneling-view"
+msgid "To use split tunneling please enable “Full disk access” for “Mullvad VPN” in the macOS system settings."
+msgstr ""
+
 #. Error message showed in a dialog when an application fails to launch.
 msgctxt "split-tunneling-view"
 msgid "Unable to launch selection. %(detailedErrorMessage)s"
diff --git a/gui/src/main/daemon-rpc.ts b/gui/src/main/daemon-rpc.ts
index c86bed047f13..8cea0d4008a6 100644
--- a/gui/src/main/daemon-rpc.ts
+++ b/gui/src/main/daemon-rpc.ts
@@ -438,6 +438,13 @@ export class DaemonRpc extends GrpcClient {
     await this.callBool(this.client.setSplitTunnelState, enabled);
   }
 
+  public async needFullDiskPermissions(): Promise<boolean> {
+    const needFullDiskPermissions = await this.callEmpty<BoolValue>(
+      this.client.needFullDiskPermissions,
+    );
+    return needFullDiskPermissions.getValue();
+  }
+
   public async checkVolumes(): Promise<void> {
     await this.callEmpty(this.client.checkVolumes);
   }
diff --git a/gui/src/main/index.ts b/gui/src/main/index.ts
index 400fe39d2ad7..c9067c78e218 100644
--- a/gui/src/main/index.ts
+++ b/gui/src/main/index.ts
@@ -832,6 +832,9 @@ class ApplicationMain
       splitTunneling!.removeApplicationFromCache(application);
       return Promise.resolve();
     });
+    IpcMainEventChannel.macOsSplitTunneling.handleNeedFullDiskPermissions(() => {
+      return this.daemonRpc.needFullDiskPermissions();
+    });
 
     IpcMainEventChannel.app.handleQuit(() => this.disconnectAndQuit());
     IpcMainEventChannel.app.handleOpenUrl(async (url) => {
diff --git a/gui/src/renderer/app.tsx b/gui/src/renderer/app.tsx
index d36c27aa14c8..ddbb43aab7b1 100644
--- a/gui/src/renderer/app.tsx
+++ b/gui/src/renderer/app.tsx
@@ -345,6 +345,8 @@ export default class AppRenderer {
     IpcRendererEventChannel.splitTunneling.addApplication(application);
   public forgetManuallyAddedSplitTunnelingApplication = (application: ISplitTunnelingApplication) =>
     IpcRendererEventChannel.splitTunneling.forgetManuallyAddedApplication(application);
+  public needFullDiskPermissions = () =>
+    IpcRendererEventChannel.macOsSplitTunneling.needFullDiskPermissions();
   public setObfuscationSettings = (obfuscationSettings: ObfuscationSettings) =>
     IpcRendererEventChannel.settings.setObfuscationSettings(obfuscationSettings);
   public setEnableDaita = (value: boolean) =>
diff --git a/gui/src/renderer/components/SmallButton.tsx b/gui/src/renderer/components/SmallButton.tsx
index e88d71995248..c91fbbdb204c 100644
--- a/gui/src/renderer/components/SmallButton.tsx
+++ b/gui/src/renderer/components/SmallButton.tsx
@@ -53,6 +53,10 @@ const StyledSmallButton = styled.button<StyledSmallButtonProps>(smallText, (prop
     alignItems: 'center',
     justifyContent: 'center',
 
+    '&&:not(& + &&)': {
+      marginLeft: '0px',
+    },
+
     [`${SmallButtonGroupStart} &&`]: {
       marginLeft: 0,
       marginRight: `${BUTTON_GROUP_GAP}px`,
diff --git a/gui/src/renderer/components/SplitTunnelingSettings.tsx b/gui/src/renderer/components/SplitTunnelingSettings.tsx
index ed999ba867a5..7e8830e6f89d 100644
--- a/gui/src/renderer/components/SplitTunnelingSettings.tsx
+++ b/gui/src/renderer/components/SplitTunnelingSettings.tsx
@@ -43,6 +43,7 @@ import {
   StyledPageCover,
   StyledSearchBar,
   StyledSpinnerRow,
+  StyledSystemSettingsButton,
 } from './SplitTunnelingSettingsStyles';
 import Switch from './Switch';
 
@@ -313,9 +314,12 @@ export function SplitTunnelingSettings(props: IPlatformSplitTunnelingSettingsPro
     removeSplitTunnelingApplication,
     forgetManuallyAddedSplitTunnelingApplication,
     getSplitTunnelingApplications,
+    needFullDiskPermissions,
     setSplitTunnelingState,
   } = useAppContext();
-  const splitTunnelingEnabled = useSelector((state: IReduxState) => state.settings.splitTunneling);
+  const splitTunnelingEnabledValue = useSelector(
+    (state: IReduxState) => state.settings.splitTunneling,
+  );
   const splitTunnelingApplications = useSelector(
     (state: IReduxState) => state.settings.splitTunnelingApplications,
   );
@@ -323,6 +327,23 @@ export function SplitTunnelingSettings(props: IPlatformSplitTunnelingSettingsPro
   const [searchTerm, setSearchTerm] = useState('');
   const [applications, setApplications] = useState<ISplitTunnelingApplication[]>();
 
+  const [splitTunnelingAvailable, setSplitTunnelingAvailable] = useState(
+    window.env.platform === 'darwin' ? undefined : true,
+  );
+
+  const splitTunnelingEnabled = splitTunnelingEnabledValue && (splitTunnelingAvailable ?? false);
+
+  const fetchNeedFullDiskPermissions = useCallback(async () => {
+    const needPermissions = await needFullDiskPermissions();
+    setSplitTunnelingAvailable(!needPermissions);
+  }, [needFullDiskPermissions]);
+
+  useEffect((): void | (() => void) => {
+    if (window.env.platform === 'darwin') {
+      void fetchNeedFullDiskPermissions();
+    }
+  }, [fetchNeedFullDiskPermissions]);
+
   const onMount = useEffectEvent(async () => {
     const { fromCache, applications } = await getSplitTunnelingApplications();
     setApplications(applications);
@@ -441,14 +462,25 @@ export function SplitTunnelingSettings(props: IPlatformSplitTunnelingSettingsPro
       <SettingsHeader>
         <StyledHeaderTitleContainer>
           <StyledHeaderTitle>{strings.splitTunneling}</StyledHeaderTitle>
-          <Switch isOn={splitTunnelingEnabled} onChange={setSplitTunnelingState} />
+          <Switch
+            isOn={splitTunnelingEnabled}
+            disabled={!splitTunnelingAvailable}
+            onChange={setSplitTunnelingState}
+          />
         </StyledHeaderTitleContainer>
-        <HeaderSubTitle>
-          {messages.pgettext(
-            'split-tunneling-view',
-            'Choose the apps you want to exclude from the VPN tunnel.',
-          )}
-        </HeaderSubTitle>
+        <MacOsSplitTunnelingAvailability
+          needFullDiskPermissions={
+            window.env.platform === 'darwin' && splitTunnelingAvailable === false
+          }
+        />
+        {splitTunnelingAvailable ? (
+          <HeaderSubTitle>
+            {messages.pgettext(
+              'split-tunneling-view',
+              'Choose the apps you want to exclude from the VPN tunnel.',
+            )}
+          </HeaderSubTitle>
+        ) : null}
       </SettingsHeader>
 
       {splitTunnelingEnabled && (
@@ -495,6 +527,34 @@ export function SplitTunnelingSettings(props: IPlatformSplitTunnelingSettingsPro
   );
 }
 
+interface MacOsSplitTunnelingAvailabilityProps {
+  needFullDiskPermissions: boolean;
+}
+
+function MacOsSplitTunnelingAvailability({
+  needFullDiskPermissions,
+}: MacOsSplitTunnelingAvailabilityProps) {
+  const { showFullDiskAccessSettings } = useAppContext();
+
+  return (
+    <>
+      {needFullDiskPermissions === true ? (
+        <>
+          <HeaderSubTitle>
+            {messages.pgettext(
+              'split-tunneling-view',
+              'To use split tunneling please enable “Full disk access” for “Mullvad VPN” in the macOS system settings.',
+            )}
+          </HeaderSubTitle>
+          <StyledSystemSettingsButton onClick={showFullDiskAccessSettings}>
+            Open System Settings
+          </StyledSystemSettingsButton>
+        </>
+      ) : null}
+    </>
+  );
+}
+
 interface IApplicationListProps<T extends IApplication> {
   applications: T[] | undefined;
   rowRenderer: (application: T) => React.ReactElement;
diff --git a/gui/src/renderer/components/SplitTunnelingSettingsStyles.tsx b/gui/src/renderer/components/SplitTunnelingSettingsStyles.tsx
index 1aea5108a14b..a2019fba8dcc 100644
--- a/gui/src/renderer/components/SplitTunnelingSettingsStyles.tsx
+++ b/gui/src/renderer/components/SplitTunnelingSettingsStyles.tsx
@@ -8,6 +8,7 @@ import ImageView from './ImageView';
 import { NavigationScrollbars } from './NavigationBar';
 import SearchBar from './SearchBar';
 import { HeaderTitle } from './SettingsHeader';
+import { SmallButton } from './SmallButton';
 
 export const StyledPageCover = styled.div<{ $show: boolean }>((props) => ({
   position: 'absolute',
@@ -122,3 +123,8 @@ export const StyledSearchBar = styled(SearchBar)({
   marginRight: measurements.viewMargin,
   marginBottom: measurements.buttonVerticalMargin,
 });
+
+export const StyledSystemSettingsButton = styled(SmallButton)({
+  width: '100%',
+  marginTop: '24px',
+});
diff --git a/gui/src/shared/ipc-schema.ts b/gui/src/shared/ipc-schema.ts
index 954dce168014..a2282e28498b 100644
--- a/gui/src/shared/ipc-schema.ts
+++ b/gui/src/shared/ipc-schema.ts
@@ -240,6 +240,9 @@ export const ipcSchema = {
     getApplications: invoke<void, ILinuxSplitTunnelingApplication[]>(),
     launchApplication: invoke<ILinuxSplitTunnelingApplication | string, LaunchApplicationResult>(),
   },
+  macOsSplitTunneling: {
+    needFullDiskPermissions: invoke<void, boolean>(),
+  },
   splitTunneling: {
     '': notifyRenderer<ISplitTunnelingApplication[]>(),
     setState: invoke<boolean, void>(),