Question about prepping RNA-Seq samples #447
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ACharbonneau
josesanchez1815
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RNA - Seq
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These questions came up as we were discussing slides 25-26 of 91 from these slides https://osf.io/kj5av/ Does the PCR amplify the strands with adapters on it? |
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Answered by
ACharbonneau
Jul 27, 2021
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Different robots have different abilities so that's hard to answer. For the PCR, yes. You make sticky ends on your fragmented sequences, ligate on adaptors, and those adaptors also serve as the PCR primer annealing locations |
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ACharbonneau
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Different robots have different abilities so that's hard to answer.
For the PCR, yes. You make sticky ends on your fragmented sequences, ligate on adaptors, and those adaptors also serve as the PCR primer annealing locations