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unable to untangle injected graph #543

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michael-olufemi opened this issue Jan 4, 2024 · 3 comments
Open

unable to untangle injected graph #543

michael-olufemi opened this issue Jan 4, 2024 · 3 comments

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@michael-olufemi
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michael-olufemi commented Jan 4, 2024

Hello,

when I try to untangle a graph with injected gene annotations, I get this error
[odgi::algorithms::stepindex] unable to index empty path CNV1
commands used is
odgi untangle -i inject.cnv.og -r NAM#1#chrI:32999-44495 -j 0.5 -t 4 -g | less

I get this error when I use this command
odgi untangle -i inject.cnv.og -r CNV1 -j 0.5 -t 4 -g | less

[odgi::untangle] error: no path CNV1 found in graph.

when I do this
odgi paths -i inject.cnv.og -L | less
The path is represent and I can see it when I visualize it

Thanks for the help

@michael-olufemi
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michael-olufemi commented Jan 4, 2024

I see the problem now,
when I do this
odgi paths -i inject.cnv.og -f > inject.fa | grep 'CNV1'
It produces no sequence
When I ran odgi inject, it prodeuces no error.
Why is the path empty?

Thanks

@ekg
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ekg commented Jan 4, 2024

That is strange. What's it look like in the GFA?

@michael-olufemi
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Hello @ekg,
I realized that the coordinates I provided during odgi inject is wrong. I corrected it and this command below works now
odgi untangle -i inject.cnv.og -r NAM#1#chrI:32999-44495 -j 0.5 -t 4 -g | less
However, this is still not working
odgi untangle -i inject.cnv.og -r CNV1 -j 0.5 -t 4 -g | less

[odgi::untangle] error: no path CNV1 found in graph.

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