diff --git a/include/rtc/h264rtpdepacketizer.hpp b/include/rtc/h264rtpdepacketizer.hpp
index bf4505687..d36a043bc 100644
--- a/include/rtc/h264rtpdepacketizer.hpp
+++ b/include/rtc/h264rtpdepacketizer.hpp
@@ -15,6 +15,7 @@
 #include "common.hpp"
 #include "mediahandler.hpp"
 #include "message.hpp"
+#include "nalunit.hpp"
 #include "rtp.hpp"
 
 #include <iterator>
@@ -24,14 +25,18 @@ namespace rtc {
 /// RTP depacketization for H264
 class RTC_CPP_EXPORT H264RtpDepacketizer : public MediaHandler {
 public:
-	H264RtpDepacketizer() = default;
+	using Separator = NalUnit::Separator;
+
+	H264RtpDepacketizer(Separator separator = Separator::LongStartSequence);
 	virtual ~H264RtpDepacketizer() = default;
 
 	void incoming(message_vector &messages, const message_callback &send) override;
 
 private:
 	std::vector<message_ptr> mRtpBuffer;
+	const NalUnit::Separator separator;
 
+	void addSeparator(binary& accessUnit);
 	message_vector buildFrames(message_vector::iterator firstPkt, message_vector::iterator lastPkt,
 	                           uint32_t timestamp);
 };
diff --git a/src/h264rtpdepacketizer.cpp b/src/h264rtpdepacketizer.cpp
index 79e554478..977a010ea 100644
--- a/src/h264rtpdepacketizer.cpp
+++ b/src/h264rtpdepacketizer.cpp
@@ -15,11 +15,44 @@
 
 namespace rtc {
 
-const binary naluStartCode = {byte{0}, byte{0}, byte{1}};
+const binary naluLongStartCode = {byte{0}, byte{0}, byte{0}, byte{1}};
+const binary naluShortStartCode = {byte{0}, byte{0}, byte{1}};
 
 const uint8_t naluTypeSTAPA = 24;
 const uint8_t naluTypeFUA = 28;
 
+H264RtpDepacketizer::H264RtpDepacketizer(Separator separator) : separator(separator) {
+	switch (separator) {
+	case Separator::StartSequence: [[fallthrough]];
+	case Separator::LongStartSequence: [[fallthrough]];
+	case Separator::ShortStartSequence:
+		break;
+	case Separator::Length: [[fallthrough]];
+	default:
+		throw std::invalid_argument("Invalid separator");
+	}
+}
+
+void H264RtpDepacketizer::addSeparator(binary& accessUnit)
+{
+	switch (separator) {
+	case Separator::StartSequence: [[fallthrough]];
+	case Separator::LongStartSequence:
+		accessUnit.insert(accessUnit.end(),
+		                  naluLongStartCode.begin(),
+		                  naluLongStartCode.end());
+		break;
+	case Separator::ShortStartSequence:
+		accessUnit.insert(accessUnit.end(),
+		                  naluShortStartCode.begin(),
+		                  naluShortStartCode.end());
+		break;
+	case Separator::Length: [[fallthrough]];
+	default:
+		throw std::invalid_argument("Invalid separator");
+	}
+}
+
 message_vector H264RtpDepacketizer::buildFrames(message_vector::iterator begin,
                                                 message_vector::iterator end, uint32_t timestamp) {
 	message_vector out = {};
@@ -49,8 +82,7 @@ message_vector H264RtpDepacketizer::buildFrames(message_vector::iterator begin,
 			    std::to_integer<uint8_t>(pkt->at(rtpHeaderSize + sizeof(NalUnitHeader)))};
 
 			if (nFrags++ == 0) {
-				accessUnit.insert(accessUnit.end(), naluStartCode.begin(), naluStartCode.end());
-
+				addSeparator(accessUnit);
 				accessUnit.emplace_back(
 				    byte(nalUnitHeader.idc() | nalUnitFragmentHeader.unitType()));
 			}
@@ -60,7 +92,7 @@ message_vector H264RtpDepacketizer::buildFrames(message_vector::iterator begin,
 			                      sizeof(NalUnitFragmentHeader),
 			                  pkt->end());
 		} else if (nalUnitHeader.unitType() > 0 && nalUnitHeader.unitType() < 24) {
-			accessUnit.insert(accessUnit.end(), naluStartCode.begin(), naluStartCode.end());
+			addSeparator(accessUnit);
 			accessUnit.insert(accessUnit.end(), pkt->begin() + rtpHeaderSize, pkt->end());
 		} else if (nalUnitHeader.unitType() == naluTypeSTAPA) {
 			auto currOffset = rtpHeaderSize + sizeof(NalUnitHeader);
@@ -75,7 +107,7 @@ message_vector H264RtpDepacketizer::buildFrames(message_vector::iterator begin,
 					throw std::runtime_error("H264 STAP-A declared size is larger than buffer");
 				}
 
-				accessUnit.insert(accessUnit.end(), naluStartCode.begin(), naluStartCode.end());
+				addSeparator(accessUnit);
 				accessUnit.insert(accessUnit.end(), pkt->begin() + currOffset,
 				                  pkt->begin() + currOffset + naluSize);