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anchor heatmap did not run #46

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chstellar opened this issue Sep 15, 2023 · 2 comments
Open

anchor heatmap did not run #46

chstellar opened this issue Sep 15, 2023 · 2 comments

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@chstellar
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chstellar commented Sep 15, 2023

Hi, I have encountered a problem in running SPLASH in heatmap mode. I have run SPLASH successfully and I want to visualise the results using heatmap. My input was:
nextflow run kaitlinchaung/nomad -r main -latest --input sheet.csv --element_annotations_samplesheet ../samplesheet.csv --run_anchor_heatmaps true --results_dir <results_dir> --use_heatmap_anchor_list true --heatmap_anchor_list anchor.tsv
but the pipeline ends in seconds and I saw no update the result folder:
[d8/537650] process > RUN_NOMAD:ANCHOR_HEATMAPS (h... [100%] 1 of 1 ✔ <results_dir> -[salzmanlab/nomad] Pipeline completed successfully-
I also tried to add a command that should induce an error message:
nextflow run kaitlinchaung/nomad -r main -latest --input sheet.csv --element_annotations_samplesheet ../samplesheet.csv --run_anchor_heatmaps true --results_dir <results_dir> --use_heatmap_anchor_list true --heatmap_anchor_list anchor.tsv **--additional_summary test**
but again the pipeline run without an error but gave no output. Could you please help with this?

@kaitlinchaung
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Hi @chstellar,

The anchor heatmaps should be output in "${params.results_dir}/anchor_heatmaps". It won't be located in ${params.outdir}.

Also, we recommend using the newer version of the codebase here: https://github.com/refresh-bio/SPLASH

@chstellar
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There was no directory named anchor_heatmaps generated in the designated results_dir... But I will check SPLASH2 for my analysis. Thanks!

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