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Originally posted by YidaZhang0628 April 14, 2023
I am planning to use Seurat V5 on a MERFISH dataset. The tutorial uses LoadVizgen function to read the files. However, I don't have hdf5r files from segmentation. Therefore, I won't be able to use LoadVizgen. I can read the data using ReadVizgen but it results in a plain list instead of a Seurat object. Any idea on how to read MERFISH data without segmentation information into a Seurat object?
The text was updated successfully, but these errors were encountered:
For Cellpose output, I submitted a PR handle Cellpose output #7162
See also this #7080
You may it devtools::install_github(repo = "alikhuseynov/seurat", ref = "feat/vizgen")
To load Vizgen this workflow could help make Seurat objects
Discussed in #7170
Originally posted by YidaZhang0628 April 14, 2023
I am planning to use Seurat V5 on a MERFISH dataset. The tutorial uses
LoadVizgen
function to read the files. However, I don't have hdf5r files from segmentation. Therefore, I won't be able to useLoadVizgen
. I can read the data usingReadVizgen
but it results in a plain list instead of a Seurat object. Any idea on how to read MERFISH data without segmentation information into a Seurat object?The text was updated successfully, but these errors were encountered: