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Hi,
I was wondering whether for the counting part there is a possibility to use SE BC fastq (read1) and supply a UMI fastq (read2)?
In addition, a few things are unclear to me regarding the workflow:
is UMI collapsing happening per BC-UMI or across BCs per UMI? If the same UMI is associated with multiple BCs, how is this being handled?
Does it require a perfect match between the association file BC sequences and the ones observed in read1 fastq?
Related to 2), are the base qualities somehow taken into account?
Thank you a lot!
The text was updated successfully, but these errors were encountered:
Hi,
I was wondering whether for the counting part there is a possibility to use SE BC fastq (read1) and supply a UMI fastq (read2)?
In addition, a few things are unclear to me regarding the workflow:
Thank you a lot!
The text was updated successfully, but these errors were encountered: