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Count SE BC + UMI data #76

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zkliesmete opened this issue Apr 11, 2023 · 0 comments
Open

Count SE BC + UMI data #76

zkliesmete opened this issue Apr 11, 2023 · 0 comments

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@zkliesmete
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Hi,
I was wondering whether for the counting part there is a possibility to use SE BC fastq (read1) and supply a UMI fastq (read2)?
In addition, a few things are unclear to me regarding the workflow:

  1. is UMI collapsing happening per BC-UMI or across BCs per UMI? If the same UMI is associated with multiple BCs, how is this being handled?
  2. Does it require a perfect match between the association file BC sequences and the ones observed in read1 fastq?
  3. Related to 2), are the base qualities somehow taken into account?

Thank you a lot!

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