Computer Specifications

[Kav57]

I am interested in using UNCALLED, can you give me an idea of the specification of computer required to run this to exclude the human genome in real time? Specifically, is a GPU required? Which Nanopore devices is it compatible with?

[skovaka]

UNCALLED does not require a GPU, and it's compatible with MinION and GridION. However, unfortunately we currently can't handle the full human genome, as we are limited to sequences less than ~1Gbp. I recommend you look into ReadFish (https://github.com/LooseLab/readfish), or the "adaptive sampling" option built into MinKNOW. A GPU is recommended for those methods, but you may be able to get away with a high-end CPU.