diff --git a/README.md b/README.md
index 2b350f6d..242c9201 100644
--- a/README.md
+++ b/README.md
@@ -251,12 +251,13 @@ will emulate the real IntersectionObserver, allowing you to validate that your
 components are behaving as expected.
 
 | Method                                        | Description                                                                                                                                                                       |
-| --------------------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
+|-----------------------------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
 | `mockAllIsIntersecting(isIntersecting)`       | Set `isIntersecting` on all current Intersection Observer instances. The value of `isIntersecting` should be either a `boolean` or a threshold between 0 and 1.                   |
 | `mockIsIntersecting(element, isIntersecting)` | Set `isIntersecting` for the Intersection Observer of a specific `element`. The value of `isIntersecting` should be either a `boolean` or a threshold between 0 and 1.            |
 | `intersectionMockInstance(element)`           | Call the `intersectionMockInstance` method with an element, to get the (mocked) `IntersectionObserver` instance. You can use this to spy on the `observe` and`unobserve` methods. |
 | `setupIntersectionMocking(mockFn)`            | Mock the `IntersectionObserver`, so we can interact with them in tests - Should be called in `beforeEach`. (**Done automatically in Jest environment**)                           |
-| `resetIntersectionMocking()`                  | Reset the mocks on `IntersectionObserver` - Should be called in `afterEach`. (**Done automatically in Jest environment**)                                                         |
+| `resetIntersectionMocking()`                  | Reset the mocks on `IntersectionObserver` - Should be called in `afterEach`. (**Done automatically in Jest/Vitest environment**)                                                  |
+| `destroyIntersectionMocking()`                | Destroy the mocked `IntersectionObserver` function, and return `window.IntersectionObserver` to the original browser implementation                                               |
 
 ### Testing Libraries
 
diff --git a/src/__tests__/hooks.test.tsx b/src/__tests__/hooks.test.tsx
index 6c3c5107..8d307b35 100644
--- a/src/__tests__/hooks.test.tsx
+++ b/src/__tests__/hooks.test.tsx
@@ -2,6 +2,7 @@ import { render, screen } from "@testing-library/react";
 import React, { useCallback } from "react";
 import { type IntersectionOptions, defaultFallbackInView } from "../index";
 import {
+  destroyIntersectionMocking,
   intersectionMockInstance,
   mockAllIsIntersecting,
   mockIsIntersecting,
@@ -342,6 +343,7 @@ test("should set intersection ratio as the largest threshold smaller than trigge
 });
 
 test("should handle fallback if unsupported", () => {
+  destroyIntersectionMocking();
   // @ts-ignore
   window.IntersectionObserver = undefined;
   const { rerender } = render(
@@ -363,6 +365,7 @@ test("should handle fallback if unsupported", () => {
 });
 
 test("should handle defaultFallbackInView if unsupported", () => {
+  destroyIntersectionMocking();
   // @ts-ignore
   window.IntersectionObserver = undefined;
   defaultFallbackInView(true);
@@ -383,3 +386,12 @@ test("should handle defaultFallbackInView if unsupported", () => {
     `[TypeError: IntersectionObserver is not a constructor]`,
   );
 });
+
+test("should restore the browser IntersectingObserver", () => {
+  expect(vi.isMockFunction(window.IntersectionObserver)).toBe(true);
+  destroyIntersectionMocking();
+
+  // This should restore the original IntersectionObserver
+  expect(window.IntersectionObserver).toBeDefined();
+  expect(vi.isMockFunction(window.IntersectionObserver)).toBe(false);
+});
diff --git a/src/test-utils.ts b/src/test-utils.ts
index 71339511..3be8ee9b 100644
--- a/src/test-utils.ts
+++ b/src/test-utils.ts
@@ -11,26 +11,32 @@ let isMocking = false;
 
 const observers = new Map<IntersectionObserver, Item>();
 
+// Store a reference to the original `IntersectionObserver` so we can restore it later.
+// This can be relevant if testing in a browser environment, where you actually have a native `IntersectionObserver`.
+const originalIntersectionObserver =
+  typeof window !== "undefined" ? window.IntersectionObserver : undefined;
+
 /*
  ** If we are running in a valid testing environment, we can automate mocking the IntersectionObserver.
  */
 if (
   typeof window !== "undefined" &&
   typeof beforeAll !== "undefined" &&
+  typeof beforeEach !== "undefined" &&
   typeof afterEach !== "undefined"
 ) {
-  beforeAll(() => {
-    // Use the exposed mock function. Currently, only supports Jest (`jest.fn`) and Vitest with globals (`vi.fn`).
+  const initMocking = () => {
+    // Use the exposed mock function. Currently, it supports Jest (`jest.fn`) and Vitest with globals (`vi.fn`).
     // @ts-ignore
     if (typeof jest !== "undefined") setupIntersectionMocking(jest.fn);
     else if (typeof vi !== "undefined") {
       setupIntersectionMocking(vi.fn);
     }
-  });
+  };
 
-  afterEach(() => {
-    resetIntersectionMocking();
-  });
+  beforeAll(initMocking);
+  beforeEach(initMocking);
+  afterEach(resetIntersectionMocking);
 }
 
 function getActFn() {
@@ -76,6 +82,7 @@ afterEach(() => {
  * @param mockFn The mock function to use. Defaults to `vi.fn`.
  */
 export function setupIntersectionMocking(mockFn: typeof vi.fn) {
+  if (isMocking) return;
   window.IntersectionObserver = mockFn((cb, options = {}) => {
     const item = {
       callback: cb,
@@ -122,6 +129,17 @@ export function resetIntersectionMocking() {
   observers.clear();
 }
 
+/**
+ * Destroy the IntersectionObserver mock function, and restore the original browser implementation of `IntersectionObserver`.
+ * You can use this to opt of mocking in a specific test.
+ **/
+export function destroyIntersectionMocking() {
+  resetIntersectionMocking();
+  // @ts-ignore
+  window.IntersectionObserver = originalIntersectionObserver;
+  isMocking = false;
+}
+
 function triggerIntersection(
   elements: Element[],
   trigger: boolean | number,