diff --git a/doc/changes/devel/12481.bugfix.rst b/doc/changes/devel/12481.bugfix.rst
new file mode 100644
index 00000000000..a9108fe4040
--- /dev/null
+++ b/doc/changes/devel/12481.bugfix.rst
@@ -0,0 +1 @@
+- Fix reading segmented recordings with :func:`mne.io.read_raw_eyelink` by `Dominik Welke`_.
\ No newline at end of file
diff --git a/mne/io/eyelink/_utils.py b/mne/io/eyelink/_utils.py
index cefc184c2f9..99c1e1c96f6 100644
--- a/mne/io/eyelink/_utils.py
+++ b/mne/io/eyelink/_utils.py
@@ -507,7 +507,7 @@ def _adjust_times(
         np.arange(first, last + step / 2, step), columns=[time_col]
     )
     return pd.merge_asof(
-        new_times, df, on=time_col, direction="nearest", tolerance=step / 10
+        new_times, df, on=time_col, direction="nearest", tolerance=step / 2
     )