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cross-domain interactions #255

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gastronaut82 opened this issue Mar 17, 2024 · 1 comment
Open

cross-domain interactions #255

gastronaut82 opened this issue Mar 17, 2024 · 1 comment

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@gastronaut82
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Hi there,

I'm trying to run a cross-domain analysis using ITS2 data (2 fungal taxa) and 16s data, using:
spiec_result_gr1 <- multi.spiec.easi(list(counts_hmp216S, counts_hmp2prot), method='mb', nlambda=40, lambda.min.ratio=1e-2,
pulsar.params = list(thresh = 0.05))

However, I keep getting the following error. I've tried filtering in multiple ways, but the same thing keeps happening.
How to fix this?
Secondly, is this supposed to be performed only on count data, or on compositional i.e. relative abundance data?

ERROR message:

Applying data transformations...
Error in if (max < min) stop("Did you flip min and max?") :
missing value where TRUE/FALSE needed
In addition: Warning message:
In spiec.easi.list(datalist, method = method, sel.criterion = sel.criterion, :
input list contains data of mixed classes.

@gastronaut82
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Nevermind - I figured this problem out - it was because of columns that contained zeroes.

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