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Update README.md
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almart7 authored Nov 27, 2023
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Expand Up @@ -33,9 +33,7 @@ The package is distributed as a compressed file containing the proper structure.
The workflow uses different programs to generate the information and although many of them will be automatically installed by conda, there are some not present and need to be manually installed:

- **UTRScan**: currently bundled with isoannot.
- **Interproscan**: follow instructions in https://interproscan-docs.readthedocs.io/en/latest/HowToDownload.html. By default, IsoAnnot assumes that interproscan.sh is located in the subfolder "software/interproscan/". If you have it installed in another directory, you can always create a symbolic link or modify the parameter "interproscan_path" inside the configuration file "config/generic/config.yaml".

Please take into account that you will need to activate SignalP and TMHMM https://interproscan-docs.readthedocs.io/en/latest/ActivatingLicensedAnalyses.html. For SignalP, you will also have to modify the signalP configuration file "bin/signalp/4.1/signalp" and specify its absolute path in your system.
- **InterProScan**: you can install InterProScan using the InterproScan.sh script we provide within IsoAnnot. By default, IsoAnnot assumes that interproscan.sh is located in the subfolder "software/interproscan/". If you have it installed in another directory, you can always create a symbolic link or modify the parameter "interproscan_path" inside the configuration file "config/generic/config.yaml". Please take into account that you will need to activate SignalP and TMHMM by following the installation script instructions.

- **PhosphoSitePlus** files: IsoAnnot retrieves information regarding post traductional modifications from the PhosphoSitePlus database.
The necessary files are included in the IsoAnnot compressed file, but you will have to download them manually if you want to update them: https://www.phosphosite.org/staticDownloads
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