Sofie Thijs ([email protected])
Here you will find the outline, daily schedule, and powerpoint presentations for the practicum conjugation.
| Time | Activity | Room |
|---|---|---|
| Thu 22/10 | ||
| 8h45-9h00 | Biosafety presentation: watch before you come to the class | online |
| 9h00-9h30 | Introduction to the practicum | G2 |
| 9h30-12h00 | Genomic DNA extraction | G42 |
| 12h00-13h00 | Lunch Break | |
| 13h00-14h45 | Plasmid DNA extraction | G42 |
| 15h00-16h00 | Gel-electrophoresis of gDNA and plasmid DNA | G42 |
| 16h00-16h15 | Cleaning | G42 |
| Fri 23/10 | ||
| 9h00-11h30 | Perform a bacterial conjugaison | G41 |
| 11h30-14h40 | Lunch break & plate incubation | |
| 14h30-16h00 | Plating of the conjugation mixture on a selective medium to isolate the transconjugant | G41 |
| ------------- | ------------- | ------------- |
By the end of the practicum, you should be able to:
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Conduct, plan and execute an experiment to extract genomic DNA and plasmid DNA from a bacterial strain, and visualize the results on an agarose gel.
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Derive hypotheses for the differences in genome organization (presence of plasmid, yes or no) based on observations of the organisms environment, and lifestyle.
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Explore horizontal gene transfer in bacterial cultures, by transfer of the plasmid encoding for the green-fluorescent protein, between bacteria.
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To develop skills in molecular biology, and sterile techniques for handling bacterial cultures.
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Interpret and compare gel results with fluorescence microscopy images, gfp-expression and the working of a fluorescence microscope.
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Learn how to report against report guidelines.