v0.7

PacificBiosciences · May 9, 2023 · 037797d · 037797d
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diff --git a/CHANGELOG.md b/CHANGELOG.md
@@ -1,3 +1,8 @@
+# v0.7 changelog
+* New feature to limit reads to N reads (`--downsample`) in case of samples with extremely high depth.
+* Updated Qiime2 to 2023.2 version (This should not affect downstream results) to get rid of
+annoying installation issue with old Qiime2 version.
+
 # v0.5 changelog
 * Allow splitting samples into group for dada2 noise (pool column in metadata TSV, see
   GitHub documentation [here](https://github.com/PacificBiosciences/pb-16S-nf#pooling)).

diff --git a/README.md b/README.md
@@ -81,6 +81,7 @@ nextflow run main.nf --help
   --front_p    Forward primer sequence. Default to F27. (default: AGRGTTYGATYMTGGCTCAG)
   --adapter_p    Reverse primer sequence. Default to R1492. (default: AAGTCGTAACAAGGTARCY)
   --filterQ    Filter input reads above this Q value (default: 20).
+  --downsample    Limit reads to a maximum of N reads if there are more than N reads (default: off)
   --max_ee    DADA2 max_EE parameter. Reads with number of expected errors higher than
               this value will be discarded (default: 2)
   --minQ    DADA2 minQ parameter. Reads with any base lower than this score