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# Introduction to Bulk RNA-seq data analysis
### 2nd, 3rd, 4th Oct 2024
### 12, 19, 26 Nov 2024
#### In person
#### Bioinformatics Training Facility, Craik-Marshall Building, Downing Site, University of Cambridge

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## Instructors

* Ashley Sawle (CRUK Cambridge Institute)
* Abbi Edwards (CRUK Cambridge Institute)
* Betty Wang (Dpt Clinical Neurosciences, University of Cambridge)
* Ian Tsang (NIAB)
* Jiayin Hong (Dpt Biochemistry, University of Cambridge)
* Ramy Ragheb (Stem Cell Institute, University of Cambridge)
* Chandra Chilamakuri (CRUK Cambridge Institute)
* Yuki Ye (Dpt Clinical Neurosciences, University of Cambridge)
* Erin Doody (Sainsbury Laboratory)

## Outline

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### Day 1

**Trainers:** Abbi, Betty, Ian, Ramy, Hugo
**Trainers:**

9:30 - 9:45 - [Welcome](https://docs.google.com/presentation/d/13mwQfPCpYzj0iz5EyTQHXRd1KNwfds2ILdY8EiK2020/edit?usp=sharing)! - Bajuna

9:45 - 10:15 - [Introduction to RNAseq Methods](Bulk_RNAseq_Course_Base/Markdowns/01_Introduction_to_RNAseq_Methods.html) - Betty
9:45 - 10:15 - [Introduction to RNAseq Methods](Bulk_RNAseq_Course_Base/Markdowns/01_Introduction_to_RNAseq_Methods.html) - Chandra

10:15 - 11:00 [Raw read file format and QC](Bulk_RNAseq_Course_Base/Markdowns/02_FastQC_introduction.html) - Betty
10:15 - 11:00 [Raw read file format and QC](Bulk_RNAseq_Course_Base/Markdowns/02_FastQC_introduction.html) - Erin

- [Practical](Bulk_RNAseq_Course_Base/Markdowns/02_FastQC_practical.html)
- [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/02_FastQC_solutions.html)
<!-- - [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/02_FastQC_solutions.html) -->

11:00 - 13:30 [Alignment and Quantification of Gene Expression with Salmon](Bulk_RNAseq_Course_Base/Markdowns/03_Quantification_with_Salmon_introduction.html) - Hugo
11:00 - 13:30 [Alignment and Quantification of Gene Expression with Salmon](Bulk_RNAseq_Course_Base/Markdowns/03_Quantification_with_Salmon_introduction.html) - Erin

- [Practical](Bulk_RNAseq_Course_Base/Markdowns/03_Quantification_with_Salmon_practical.html)
- [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/03_Quantification_with_Salmon_solutions.html)
<!-- - [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/03_Quantification_with_Salmon_solutions.html) -->

13:30 - 14:30 Lunch

14:30 - 15:30 [QC of alignment](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_introduction.html) - Abbi
14:30 - 15:30 [QC of alignment](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_introduction.html) - Ash

- [Practical](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_practical.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_practical.pdf))
- [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_solutions.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_solutions.pdf))
<!-- - [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_solutions.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/04_Quality_Control_solutions.pdf)) -->

15.30 - 17.30 [Data Exploration in R](Bulk_RNAseq_Course_Base/Markdowns/05_Data_Exploration_Slides.html) - Ian
15.30 - 17.30 [Data Exploration in R](Bulk_RNAseq_Course_Base/Markdowns/05_Data_Exploration_Slides.html) - Ash

- [Practical](Bulk_RNAseq_Course_Base/Markdowns/05_Data_Exploration.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/05_Data_Exploration.pdf))
<!-- - [Practical solutions](Bulk_RNAseq_Course_Base/Markdowns/05_Data_Exploration_solutions.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/05_Data_Exploration_solutions.pdf)) -->
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### Day 2

**Trainers:** Ash, Abbi (AM), Ian, Ramy
**Trainers:**

9:30 - 10:15 [Introduction to RNAseq Analysis in R](Bulk_RNAseq_Course_Base/Markdowns/06_Introduction_to_RNAseq_Analysis_in_R.html) - Abbi
9:30 - 10:15 [Introduction to RNAseq Analysis in R](Bulk_RNAseq_Course_Base/Markdowns/06_Introduction_to_RNAseq_Analysis_in_R.html) - Betty

10:15 - 11:30 [Statistical Analysis of Bulk RNAseq Data](Bulk_RNAseq_Course_Base/additional_scripts_and_materials/07_Statistics_1.pdf) - Abbi
10:15 - 11:30 [Statistical Analysis of Bulk RNAseq Data](Bulk_RNAseq_Course_Base/additional_scripts_and_materials/07_Statistics_1.pdf) - Betty

11:30 - 17:30 [Linear Models in R and DESeq2 (Slides)](Bulk_RNAseq_Course_Base/additional_scripts_and_materials/07_Statistics_2.pdf) - Abbi/Ash
11:30 - 17:30 [Linear Models in R and DESeq2 (Slides)](Bulk_RNAseq_Course_Base/additional_scripts_and_materials/07_Statistics_2.pdf) - Chandra

- [Practical - Differential Expression for RNA-seq](Bulk_RNAseq_Course_Base/Markdowns/08_DE_analysis_with_DESeq2.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/08_DE_analysis_with_DESeq2.pdf)) - Ash/Abbi
- [Practical - Differential Expression for RNA-seq](Bulk_RNAseq_Course_Base/Markdowns/08_DE_analysis_with_DESeq2.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/08_DE_analysis_with_DESeq2.pdf)) - Chandra
<!-- - [practical solutions](Bulk_RNAseq_Course_Base/Markdowns/08_DE_analysis_with_DESeq2_solutions.html) ([pdf](Bulk_RNAseq_Course_Base/Markdowns/08_DE_analysis_with_DESeq2_solutions.pdf)) -->
<!-- - [live script](live_scripts/DESeq2_script.R) -->

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### Day 3

**Trainers:** Ash, Ian, Jiayin, Ramy
**Trainers:**

9:30 - 9:45 - [Recap of Day 1 and 2](Bulk_RNAseq_Course_Base/additional_scripts_and_materials/Analysis_of_RNA-seq_data_day3recap.pdf) - Ash
9:30 - 9:45 - [Recap of Day 1 and 2](Bulk_RNAseq_Course_Base/additional_scripts_and_materials/Analysis_of_RNA-seq_data_day3recap.pdf) - Chandra

9:45 - 11:00 [Annotation of RNA-seq results](Bulk_RNAseq_Course_Base/Markdowns/09_Annotation_Slides.html) - Ash.
9:45 - 11:00 [Annotation of RNA-seq results](Bulk_RNAseq_Course_Base/Markdowns/09_Annotation_Slides.html) - Puspendu
- [Practical](Bulk_RNAseq_Course_Base/Markdowns/09_Annotation.html)
<!-- - [live script](live_scripts/visualisation.R) -->

11:00 - 12.30 [Visualisation differential expression results](Bulk_RNAseq_Course_Base/Markdowns/10_Data_Visualisation_Slides.html) - Jiayin
11:00 - 12.30 [Visualisation differential expression results](Bulk_RNAseq_Course_Base/Markdowns/10_Data_Visualisation_Slides.html) - Yuki

- [Practical](Bulk_RNAseq_Course_Base/Markdowns/10_Data_Visualisation.html)
<!-- - [practical solutions](Bulk_RNAseq_Course_Base/Markdowns/10_Data_Visualisation_solutions.html) -->
<!-- - [live script](live_scripts/visualisation.R) -->

12.30 - 13.30 Lunch

13.30 - 16:30 [Gene-set testing](Bulk_RNAseq_Course_Base/Markdowns/11_Gene_set_testing_introduction.html) - Ian
13.30 - 16:30 [Gene-set testing](Bulk_RNAseq_Course_Base/Markdowns/11_Gene_set_testing_introduction.html) - Puspendu
- [Practical (html)](Bulk_RNAseq_Course_Base/Markdowns/11_Gene_set_testing.html) [(pdf)](Bulk_RNAseq_Course_Base/Markdowns/11_Gene_set_testing.pdf)
<!-- - [Practical solutions (html)](Bulk_RNAseq_Course_Base/Markdowns/11_Gene_set_testing_solutions.html) (pdf)](Bulk_RNAseq_Course_Base/Markdowns/11_Gene_set_testing_solutions.pdf) -->
<!-- - [Live Script](live_scripts/gene_set_testing.R) -->
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