Update of README.

README.md

@@ -13,38 +13,42 @@ RESCRIPt is a python 3 package to support a variety of operations for managing a
 
 RESCRIPt will be installable as conda package in the near future. In the meantime, we provide two routes for source installation: a minimal RESCRIPt environment, or within an existing QIIME 2 environment:
 
-### Option 1: Minimal RESCRIPt environment:
-First create a conda environment and install relevant dependencies:
+### Option 1: Installed as part of a QIIME 2 distribution.
+
+For QIIME 2 versions `2023.9` and later, RESCRIPt is installed as part of the QIIME 2 "Shotgun Distribution", as outlined in the [QIIME 2 Documentation](https://docs.qiime2.org/). RESCRIPt is available as part of the "Amplicon Distrubtion" for QIIME 2 releases `2024.2` and later.
+
+### Option 2: Minimal RESCRIPt environment 
+
+To make use of the latest "minimal" RESCRIPt release, some components of QIIME 2 (releases `2023.9` and later) are required:
+
+First create a conda environment and install relevant dependencies using either `conda` or `mamba`. 
+*Note: update `{ENV_VERSION}` in the commands below to match the QIIME 2 release.*
 
 ```
 conda create -y -n rescript
 conda activate rescript
 conda install \
-  -c conda-forge -c bioconda -c qiime2 -c https://packages.qiime2.org/qiime2/2023.5/tested/ -c defaults \
-  qiime2 q2cli q2templates q2-types q2-longitudinal q2-feature-classifier 'q2-types-genomics>2023.2' \
+  -c https://packages.qiime2.org/qiime2/{ENV_VERSION}/shotgun/passed/ \
+  -c https://packages.qiime2.org/qiime2/{ENV_VERSION}/amplicon/passed/ \
+  -c conda-forge -c bioconda -c qiime2 -c defaults \
+  qiime2 q2cli q2templates q2-types q2-types-genomics q2-longitudinal q2-feature-classifier \
   "pandas>=0.25.3" xmltodict ncbi-datasets-pylib
 ```
+
 Install source:
 
 ```
 pip install git+https://github.com/bokulich-lab/RESCRIPt.git
-```
-
-### Option 2: Install within QIIME 2 environment
-First activate your QIIME 2 environment (ver 2022.8 or later) and install relevant dependencies:
 
+qiime rescript --help
 ```
-conda activate qiime2-2023.2
-conda install -c conda-forge -c bioconda -c qiime2 -c https://packages.qiime2.org/qiime2/2023.5/tested/ -c defaults \
-  xmltodict 'q2-types-genomics>2023.2' ncbi-datasets-pylib
-```
-Install source:
 
-```
-pip install git+https://github.com/bokulich-lab/RESCRIPt.git
-```
+### Prior versions of RESCRIPt / QIIME 2.
+
+For details on how to install prior versions of RESCRIPt / QIIME 2, please see the `install-prior-versions.md` document.
 
 ### Read help documentation
+
 To view a help menu for using rescript via the QIIME 2 CLI:
 ```
 qiime dev refresh-cache
@@ -59,10 +63,14 @@ These tutorials demonstrate some of the basic functionality of RESCRIPt, via the
 - [Building a COI database with BOLD sequences](https://forum.qiime2.org/t/building-a-coi-database-from-bold-references/16129)
 - [Building a COI database with NCBI sequences](https://forum.qiime2.org/t/building-a-coi-database-from-ncbi-references/16500)
 - [Using RESCRIPt's 'extract-seq-segments' to extract reference sequences without PCR primer pairs](https://forum.qiime2.org/t/using-rescripts-extract-seq-segments-to-extract-reference-sequences-without-pcr-primer-pairs/23618)
+- [How to train a GTDB SSU classifier using RESCRIPt](https://forum.qiime2.org/t/how-to-train-a-gtdb-ssu-classifier-using-rescript/25725)
+- [Constructing an RDP classifier](https://forum.qiime2.org/t/importing-sequence-data-with-lower-case-nucleotide-characters-constructing-an-rdp-classifier-as-an-example/25158)
+- [How to train a UNITE classifier using RESCRIPt](https://forum.qiime2.org/t/how-to-train-a-unite-classifier-using-rescript/28285)
 
 Examples of visualizations produced by RESCRIPt actions can be found in this [Visualization Gallery](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494#heading--seventeenth-header). Other code examples can be found [here](https://github.com/bokulich-lab/db-benchmarks-2020).
 
 ## Getting Help
+
 Problem? Suggestion? Technical errors and user support requests can be filed on the [QIIME 2 Forum](https://forum.qiime2.org/).
 
 
@@ -79,6 +87,10 @@ RESCRIPt is released under a BSD-3-Clause license. See LICENSE for more details.
 
 However, other resources accessible via RESCRIPt are released under different licenses, as detailed below.
 
-**The SILVA database** versions are released under different licenses. Refer to the [current SILVA release license information](https://www.arb-silva.de/silva-license-information/) for more details.
+**If using the SILVA database** (*e.g.*, with `get-silva-data`): Versions are released under different licenses. Refer to the [current SILVA release license information](https://www.arb-silva.de/silva-license-information/) for more details. [How to cite SILVA](https://www.arb-silva.de/contact/).
+
+**If using NCBI Genbank data** (*e.g.*, with `get-ncbi-data`): See the [NCBI disclaimer and copyright notice](https://www.ncbi.nlm.nih.gov/home/about/policies/) for more details. [How to cite NCBI](https://support.nlm.nih.gov/knowledgebase/article/KA-03391/en-us).
+
+**If using GTDB data** (*e.g.*, with `get-gtdb-data`): See the [GTDB "about" page](https://gtdb.ecogenomic.org/about) for more details. [How to cite GTDB](https://gtdb.ecogenomic.org/about).
 
-**If using NCBI Genbank data** (e.g., with `get-ncbi-data`): See the [NCBI disclaimer and copyright notice](https://www.ncbi.nlm.nih.gov/home/about/policies/)
+**If using UNITE data** (*e.g.*, with `get-unite-data`): See the [UNITE citation page](https://unite.ut.ee/cite.php) for more details. [How to cite UNITE](https://unite.ut.ee/cite.php). 

install-prior-versions.md

@@ -0,0 +1,45 @@
+# How to install prior versions of RESCRIPt.
+
+## Install within the QIIME 2 `2023.9` release: 
+
+For this version of QIIME 2, RESCRIPt is included within the [qiime2-shotgun-2023.9 distribution](https://docs.qiime2.org/2023.9/install/native/#qiime-2-shotgun-distribution). You'll be able to run your RESCRIPt commands within this environment, then switch back to [qiime2-amplicon-2023.9](https://docs.qiime2.org/2023.9/install/native/#qiime-2-amplicon-distribution) environment if needed. 
+
+It is possible to install RESCRIPt within `qiime2-amplicon-2023.9`. YOu can try via the following commands:
+
+```
+conda activate qiime2-amplicon-2023.9
+
+conda install -c conda-forge -c bioconda -c qiime2 \
+    -c https://packages.qiime2.org/qiime2/2023.9/shotgun/passed/  \
+    -c defaults   xmltodict 'q2-types-genomics>2023.5' ncbi-datasets-pylib
+```
+
+Install source:
+
+```
+pip install git+https://github.com/bokulich-lab/RESCRIPt.git
+
+qiime rescript --help
+```
+
+
+## Install within the QIIME 2 releases `2023.2` - `2023.7`.
+The typical apporoach will be something like the command below. *Make sure the qiime version within the http string matches the version of the active qiime environment.*
+
+```
+conda activate qiime2-2023.7
+
+conda install -c conda-forge -c bioconda -c qiime2 \
+    -c https://packages.qiime2.org/qiime2/2023.7/passed/ \
+    -c defaults xmltodict 'q2-types-genomics>2023.2' ncbi-datasets-pylib
+```
+Install source:
+
+```
+pip install git+https://github.com/bokulich-lab/RESCRIPt.git
+
+qiime rescript --help
+```
+
+## Install within prior releases of QIIME 2 `2022.8` and earlier.
+Download any of the prior [Releases](https://github.com/bokulich-lab/RESCRIPt/releases) and consult that version's README file for appropriate Installation instructions.