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lcolladotor committed Jul 3, 2023
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2 changes: 1 addition & 1 deletion 05_iSEE_intro.Rmd
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Expand Up @@ -12,7 +12,7 @@ library("SummarizedExperiment")
## Adapted from the official documentation:
## First we create the data pieces that we'll use to build our
## First we create the data pieces that we'll use to build our
## SummarizedExperiment object. In this case, we'll have 200 genes
## measured in 6 samples.
nrows <- 200
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4 changes: 2 additions & 2 deletions 10_limma_overview.Rmd
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Expand Up @@ -93,7 +93,7 @@ table(rse_gene_SRP045638$assigned_gene_prop < 0.3)
rse_gene_SRP045638 <- rse_gene_SRP045638[, rse_gene_SRP045638$assigned_gene_prop > 0.3]
## Lets compute the mean expression levels.
##
##
## Note: in a real analysis we would likely do this with RPKMs or CPMs instead
## of counts. That is, we would use one of the following options:
# edgeR::filterByExpr() https://bioconductor.org/packages/edgeR/ https://rdrr.io/bioc/edgeR/man/filterByExpr.html
Expand Down Expand Up @@ -204,7 +204,7 @@ From `vGene$E` we can extract the normalized expression values that `limma-voom`
## from earlier
exprs_heatmap <- vGene$E[rank(de_results$adj.P.Val) <= 50, ]
## We can now build a table with information about our samples and
## We can now build a table with information about our samples and
## then make the names a bit more friendly by making them easier to
## understand
df <- as.data.frame(colData(rse_gene_SRP045638)[, c("prenatal", "sra_attribute.RIN", "sra_attribute.sex")])
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