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Matthias Reis edited this page Sep 27, 2016 · 2 revisions

This is a client-server application to automate and control measurements using an Olympus Laser Scanning Microscope (LSM). On the client side, a python class is responsible for

  • autofocus,
  • saving/restoring microscope settings (done by class serialization),
  • choosing the appropriate laser light sources as well as
  • automating the whole measurement procedure.

Optionally, CellProfiler pipelines (www.cellprofiler.org) can be integrated to segment images during the measurement and react accordingly, if desired.

The laser scanning microscope is controlled by a vendor-supplied software, which is in turned controlled by AutoIT (https://www.autoitscript.com/site/autoit/).

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