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A wide number of bug fixes to scRNA-seq and other pipelines. In particular, many memory limits were updated.
An optional email can be sent upon pipeline completion.
The RNA-seq pipeline can now produce a fuller report upon completion if you are performing differential expression.
Sample merging in HiC works properly.
GTF files are now handled more generically, which means that they no longer need to have "_gencode" and "_ensembl" in their path.
WGBS: * Merging data from WGBS replicates is now an independent step so that dependent rules don't have to wait for successful completion of single CpG stats but can go ahead instead. * Filtering of differential methylation test results is now subject to two user-modifiable parameters minAbsDiff (default 0.2) and FDR (0.02) stored in defaults.yaml. * Metilene commandline parameters are now available in defaults.yaml. Defaults are used apart from requesting output intervals with any methylation difference (minMethDiff 0). * Additional diagnostic plots are generated - p value distribution before and after BH adjustment as well as a volcano plot. * Automatic reports are generated in every folder containing results of statistical analysis (single CpG stats, metilene DMR stats, user interval aggregate stats), as long as sample sheet is provided. * R sessionInfo() is now printed at the end of the statistical analysis.
scRNAseq: * An extention to the pipeline now takes the processed csv file from Results folder as input and runs cell filtering with a range of total transcript thresholds using monocle and subsequently runs clustering, produces tsne visualizations, calculates top 2 and top10 markers per cluster and produces heatmap visualizations for these using monocle/seurat. If the skipRaceID flag is set to False (default), all of the above are also executed using RaceID. * Stats reports were implemented for RaceID and Monocle/Seurat so that folders Filtered_cells_RaceID and Filtered_cells_monocle now contain a Stats_report.html. * User can select a metric to maximize during cell filtering (cell_filter_metric, default: gene_universe). * For calculating median GPC, RaceID counts are multiplied by the TPC threshold applied (similar to 'downscaling' in RaceID2).
all sample sheets now need to have a "name" and a "condition" column, that was not consistent before
consistent --sampleSheet [FILE] cmdl option to invoke differential analysis mode (RNA-seq, ChIP-seq, ATAC-seq), --DE/--DB were dropped