nextstrain.org/monkeypox/ingest

This is the ingest pipeline for Monkeypox virus sequences.

Usage

NOTE: All command examples assume you are within the ingest directory. If running commands from the outer monkeypox directory, please replace the . with ingest

Fetch sequences with

nextstrain build --cpus 1 . data/sequences.ndjson

Run the complete ingest pipeline with

nextstrain build --cpus 1 .

This will produce two files (within the ingest directory):

• data/metadata.tsv
• data/sequences.fasta

Run the complete ingest pipeline and upload results to AWS S3 with

nextstrain build . --configfiles config/config.yaml config/optional.yaml

Adding new sequences not from GenBank

Static Files

Do the following to include sequences from static FASTA files.

1. Convert the FASTA files to NDJSON files with:

   ./ingest/bin/fasta-to-ndjson \
       --fasta {path-to-fasta-file} \
       --fields {fasta-header-field-names} \
       --separator {field-separator-in-header} \
       --exclude {fields-to-exclude-in-output} \
       > ingest/data/{file-name}.ndjson

2. Add the following to the .gitignore to allow the file to be included in the repo:

   !ingest/data/{file-name}.ndjson

3. Add the file-name (without the .ndjson extension) as a source to ingest/config/config.yaml. This will tell the ingest pipeline to concatenate the records to the GenBank sequences and run them through the same transform pipeline.

Configuration

Configuration takes place in config/config.yaml by default. Optional configs for uploading files and Slack notifications are in config/optional.yaml.

Environment Variables

The complete ingest pipeline with AWS S3 uploads and Slack notifications uses the following environment variables:

Required

• AWS_DEFAULT_REGION
• AWS_ACCESS_KEY_ID
• AWS_SECRET_ACCESS_KEY
• SLACK_TOKEN
• SLACK_CHANNELS

Optional

These are optional environment variables used in our automated pipeline for providing detailed Slack notifications.

• GITHUB_RUN_ID - provided via github.run_id in a GitHub Action workflow
• AWS_BATCH_JOB_ID - provided via AWS Batch Job environment variables

Input data

GenBank data

GenBank sequences and metadata are fetched via NCBI Virus. The exact URL used to fetch data is constructed in bin/genbank-url.