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load PacBio data and perform sQTL analysis #75

Merged
merged 19 commits into from
Sep 17, 2021
Merged

load PacBio data and perform sQTL analysis #75

merged 19 commits into from
Sep 17, 2021

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jsaquing
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@jsaquing jsaquing commented Sep 15, 2021

additions:

  • can load PacBio transcripts and exons from LRP pipeline output
    • PacBioTranscript has SQANTI classification (FSM, ISM, NIC, NNC) as an attribute
  • PacBioTranscript and GencodeTranscript have a new many-to-one relationship indicating association between FSM/ISM transcripts and the reference transcript they match
  • ExceptionLogger helper class for reporting exceptions w/o halting entire script
  • IsoformPlot changes:
    • updated color schemes for different transcript types and pblock categories
    • isoform information can now be displayed in a table to the left of the plot
    • IsoformPlot can be drawn inside a subplot

refactoring:

  • ORF objects are now constructed from CDS intervals in gtf file instead of transcript-relative intervals in fasta headers
  • database loads from GENCODE comprehensive set
  • database only loads genes designated protein_coding
  • initialization of attributes in Protein class moved out of reconstructor methods and into cached properties

closes #71, #74

@jsaquing jsaquing requested a review from bj8th September 15, 2021 20:27
@jsaquing jsaquing added the enhancement New feature or request label Sep 17, 2021
@bj8th bj8th merged commit ab99a82 into main Sep 17, 2021
@jsaquing jsaquing linked an issue Sep 20, 2021 that may be closed by this pull request
@jsaquing jsaquing linked an issue Oct 14, 2021 that may be closed by this pull request
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