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Update dRep-pipeline.md
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tillrobin authored Oct 6, 2020
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24 changes: 3 additions & 21 deletions dRep-pipeline.md
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[TPM-normalization](#TPM-normalization)
[Basic-statistics](#Basic-statistics)

# Description

![iMGMC-pipeline](/images/pipeline.png)

# Requirements
* [BBmap](https://sourceforge.net/projects/bbmap/)
* [samtools](http://samtools.sourceforge.net/)
* [sambamba](http://lomereiter.github.io/sambamba/)
* iMGMC repository


# Data-pre-processing
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wget -O PIBAC-mMAGs.tar.gz "https://onedrive.live.com/"


**2. Index iMGMC catalog**
**2. Index PIBAC catalog**

tar -xzf iMGMC-hqMAGs-dereplicated_genomes.tar.gz
bbsplit.sh ref=iMGMC-hqMAGs-dereplicated_genomes
tar -xzf PIBAC-hqMAGs-dereplicated_genomes.tar.gz
bbsplit.sh ref=PIBAC-hqMAGs-dereplicated_genomes


# Mapping
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sortscafs=f nzo=f \
in=${SampleName}_R1_rmhost.fastq.gz \
in2=${SampleName}_R2_rmhost.fastq.gz


# TPM-normalization
You need to normalize you samples counts for a comparison. Here we use TPM-normalization to create TPM-${SampleID}.txt form ${SampleName}.covstat :

makeTPMfromCovStats.sh ${SampleName}.covstat


# Basic-statistics

under construction


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