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Add agat sp alignment output style #124
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b39835e
add help
Leila011 a3344e9
add config
Leila011 70ba7ad
add run script
Leila011 106f03d
add test data & expected output + script to fetch them
Leila011 bbafe23
config: remove required argument for boolean_true
Leila011 4544fe7
add test
Leila011 b66cf65
update changelog
Leila011 af0d1d3
run script: replace -`-verbose` by `-v`
Leila011 93e7b9e
Merge main into add-agat_sp_alignment_output_style
rcannood 5f5e7ee
Merge branch 'main' into add-agat_sp_alignment_output_style
Leila011 be9357f
add set -eo pipefail to sccript and test files
Leila011 0d9c5f1
create temporary directory and clean up on exit
Leila011 19cd3e1
update config: add requirements, update --config description
Leila011 562e86a
fix bug in test
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Original file line number | Diff line number | Diff line change | ||||
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name: agat_sp_alignment_output_style | ||||||
namespace: agat | ||||||
description: | | ||||||
The script takes a normal gtf/gff annotation format file and convert it | ||||||
to gff3 alignment format. It means it add a structure of match / | ||||||
match_part as relationship between the different features. | ||||||
keywords: [gene annotations, GFF conversion] | ||||||
links: | ||||||
homepage: https://github.com/NBISweden/AGAT | ||||||
documentation: https://agat.readthedocs.io/en/latest/tools/agat_sp_alignment_output_style.html | ||||||
issue_tracker: https://github.com/NBISweden/AGAT/issues | ||||||
repository: https://github.com/NBISweden/AGAT | ||||||
references: | ||||||
doi: 10.5281/zenodo.3552717 | ||||||
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Suggested change
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license: GPL-3.0 | ||||||
requirements: | ||||||
- commands: [agat] | ||||||
authors: | ||||||
- __merge__: /src/_authors/leila_paquay.yaml | ||||||
roles: [ author, maintainer ] | ||||||
argument_groups: | ||||||
- name: Inputs | ||||||
arguments: | ||||||
- name: --gff | ||||||
alternatives: [-g, -ref] | ||||||
description: Input GTF/GFF file. | ||||||
type: file | ||||||
required: true | ||||||
direction: input | ||||||
example: input.gff | ||||||
- name: Outputs | ||||||
arguments: | ||||||
- name: --output | ||||||
alternatives: [-o] | ||||||
description: Output GFF file. | ||||||
type: file | ||||||
direction: output | ||||||
required: true | ||||||
example: output.gff | ||||||
- name: Arguments | ||||||
arguments: | ||||||
- name: --verbose | ||||||
alternatives: [-v] | ||||||
description: Verbose option to see the warning messages when parsing the gff file. | ||||||
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Suggested change
|
||||||
type: boolean_true | ||||||
- name: --config | ||||||
alternatives: [-c] | ||||||
description: | | ||||||
AGAT config file. By default AGAT takes the original agat_config.yaml shipped with AGAT. The `--config` option gives you the possibility to use your own AGAT config file (located elsewhere or named differently). | ||||||
type: file | ||||||
required: false | ||||||
example: custom_agat_config.yaml | ||||||
resources: | ||||||
- type: bash_script | ||||||
path: script.sh | ||||||
test_resources: | ||||||
- type: bash_script | ||||||
path: test.sh | ||||||
- type: file | ||||||
path: test_data | ||||||
engines: | ||||||
- type: docker | ||||||
image: quay.io/biocontainers/agat:1.4.0--pl5321hdfd78af_0 | ||||||
setup: | ||||||
- type: docker | ||||||
run: | | ||||||
agat --version | sed 's/AGAT\s\(.*\)/agat: "\1"/' > /var/software_versions.txt | ||||||
runners: | ||||||
- type: executable | ||||||
- type: nextflow |
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```sh | ||
agat_sp_alignment_output_style.pl --help | ||
``` | ||
|
||
------------------------------------------------------------------------------ | ||
| Another GFF Analysis Toolkit (AGAT) - Version: v1.4.0 | | ||
| https://github.com/NBISweden/AGAT | | ||
| National Bioinformatics Infrastructure Sweden (NBIS) - www.nbis.se | | ||
------------------------------------------------------------------------------ | ||
|
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Name: | ||
agat_sp_alignment_output_style.pl | ||
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Description: | ||
The script takes a normal gtf/gff annotation format file and convert it | ||
to gff3 alignment format. It means it add a structure of match / | ||
match_part as relationship between the different features. | ||
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Usage: | ||
agat_sp_alignment_output_style.pl -g infile.gff [ -o outfile ] | ||
agat_sp_alignment_output_style.pl --help | ||
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Options: | ||
-g, --gff or -ref | ||
Input GTF/GFF file. | ||
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-v Verbose option to see the warning messages when parsing the gff | ||
file. | ||
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-o or --output | ||
Output GFF file. If no output file is specified, the output will | ||
be written to STDOUT. | ||
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-c or --config | ||
String - Input agat config file. By default AGAT takes as input | ||
agat_config.yaml file from the working directory if any, | ||
otherwise it takes the orignal agat_config.yaml shipped with | ||
AGAT. To get the agat_config.yaml locally type: "agat config | ||
--expose". The --config option gives you the possibility to use | ||
your own AGAT config file (located elsewhere or named | ||
differently). | ||
|
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-h or --help | ||
Display this helpful text. | ||
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Feedback: | ||
Did you find a bug?: | ||
Do not hesitate to report bugs to help us keep track of the bugs and | ||
their resolution. Please use the GitHub issue tracking system available | ||
at this address: | ||
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https://github.com/NBISweden/AGAT/issues | ||
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Ensure that the bug was not already reported by searching under Issues. | ||
If you're unable to find an (open) issue addressing the problem, open a new one. | ||
Try as much as possible to include in the issue when relevant: | ||
- a clear description, | ||
- as much relevant information as possible, | ||
- the command used, | ||
- a data sample, | ||
- an explanation of the expected behaviour that is not occurring. | ||
|
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Do you want to contribute?: | ||
You are very welcome, visit this address for the Contributing | ||
guidelines: | ||
https://github.com/NBISweden/AGAT/blob/master/CONTRIBUTING.md |
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#!/bin/bash | ||
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set -eo pipefail | ||
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## VIASH START | ||
## VIASH END | ||
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# unset flags | ||
[[ "$par_verbose" == "false" ]] && unset par_verbose | ||
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# run agat_sp_alignment_output_style.pl | ||
agat_sp_alignment_output_style.pl \ | ||
--gff "$par_gff" \ | ||
--output "$par_output" \ | ||
${par_verbose:+-v} \ | ||
${par_config:+--config "${par_config}"} |
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#!/bin/bash | ||
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set -eo pipefail | ||
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## VIASH START | ||
## VIASH END | ||
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test_dir="${meta_resources_dir}/test_data" | ||
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# create temporary directory and clean up on exit | ||
TMPDIR=$(mktemp -d "$meta_temp_dir/$meta_functionality_name-XXXXXX") | ||
function clean_up { | ||
[[ -d "$TMPDIR" ]] && rm -rf "$TMPDIR" | ||
} | ||
trap clean_up EXIT | ||
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echo "> Run $meta_name with test data" | ||
"$meta_executable" \ | ||
--gff "$test_dir/0.gff" \ | ||
--output "$TMPDIR/output.gff" | ||
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echo ">> Checking output" | ||
[ ! -f "$TMPDIR/output.gff" ] && echo "Output file output.gff does not exist" && exit 1 | ||
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echo ">> Check if output is empty" | ||
[ ! -s "$TMPDIR/output.gff" ] && echo "Output file output.gff is empty" && exit 1 | ||
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echo ">> Check if output matches expected output" | ||
diff "$TMPDIR/output.gff" "$test_dir/agat_sp_alignment_output_style_1.gff" | ||
if [ $? -ne 0 ]; then | ||
echo "Output file output.gff does not match expected output" | ||
exit 1 | ||
fi | ||
|
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echo "> Test successful" |
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##gff-version 3 | ||
scaffold625 maker gene 337818 343277 . + . ID=CLUHARG00000005458;Name=TUBB3_2 | ||
scaffold625 maker mRNA 337818 343277 . + . ID=CLUHART00000008717;Parent=CLUHARG00000005458 | ||
scaffold625 maker exon 337818 337971 . + . ID=CLUHART00000008717:exon:1404;Parent=CLUHART00000008717 | ||
scaffold625 maker exon 340733 340841 . + . ID=CLUHART00000008717:exon:1405;Parent=CLUHART00000008717 | ||
scaffold625 maker exon 341518 341628 . + . ID=CLUHART00000008717:exon:1406;Parent=CLUHART00000008717 | ||
scaffold625 maker exon 341964 343277 . + . ID=CLUHART00000008717:exon:1407;Parent=CLUHART00000008717 | ||
scaffold625 maker CDS 337915 337971 . + 0 ID=CLUHART00000008717:cds;Parent=CLUHART00000008717 | ||
scaffold625 maker CDS 340733 340841 . + 0 ID=CLUHART00000008717:cds;Parent=CLUHART00000008717 | ||
scaffold625 maker CDS 341518 341628 . + 2 ID=CLUHART00000008717:cds;Parent=CLUHART00000008717 | ||
scaffold625 maker CDS 341964 343033 . + 2 ID=CLUHART00000008717:cds;Parent=CLUHART00000008717 | ||
scaffold625 maker five_prime_UTR 337818 337914 . + . ID=CLUHART00000008717:five_prime_utr;Parent=CLUHART00000008717 | ||
scaffold625 maker three_prime_UTR 343034 343277 . + . ID=CLUHART00000008717:three_prime_utr;Parent=CLUHART00000008717 | ||
scaffold789 maker gene 558184 564780 . + . ID=CLUHARG00000003852;Name=PF11_0240 | ||
scaffold789 maker mRNA 558184 564780 . + . ID=CLUHART00000006146;Parent=CLUHARG00000003852 | ||
scaffold789 maker exon 558184 560123 . + . ID=CLUHART00000006146:exon:995;Parent=CLUHART00000006146 | ||
scaffold789 maker exon 561401 561519 . + . ID=CLUHART00000006146:exon:996;Parent=CLUHART00000006146 | ||
scaffold789 maker exon 564171 564235 . + . ID=CLUHART00000006146:exon:997;Parent=CLUHART00000006146 | ||
scaffold789 maker exon 564372 564780 . + . ID=CLUHART00000006146:exon:998;Parent=CLUHART00000006146 | ||
scaffold789 maker CDS 558191 560123 . + 0 ID=CLUHART00000006146:cds;Parent=CLUHART00000006146 | ||
scaffold789 maker CDS 561401 561519 . + 2 ID=CLUHART00000006146:cds;Parent=CLUHART00000006146 | ||
scaffold789 maker CDS 564171 564235 . + 0 ID=CLUHART00000006146:cds;Parent=CLUHART00000006146 | ||
scaffold789 maker CDS 564372 564588 . + 1 ID=CLUHART00000006146:cds;Parent=CLUHART00000006146 | ||
scaffold789 maker five_prime_UTR 558184 558190 . + . ID=CLUHART00000006146:five_prime_utr;Parent=CLUHART00000006146 | ||
scaffold789 maker three_prime_UTR 564589 564780 . + . ID=CLUHART00000006146:three_prime_utr;Parent=CLUHART00000006146 | ||
scaffold789 maker mRNA 558184 564780 . + . ID=CLUHART00000006147;Parent=CLUHARG00000003852 | ||
scaffold789 maker exon 558184 560123 . + . ID=CLUHART00000006147:exon:997;Parent=CLUHART00000006147 | ||
scaffold789 maker exon 561401 561519 . + . ID=CLUHART00000006147:exon:998;Parent=CLUHART00000006147 | ||
scaffold789 maker exon 562057 562121 . + . ID=CLUHART00000006147:exon:999;Parent=CLUHART00000006147 | ||
scaffold789 maker exon 564372 564780 . + . ID=CLUHART00000006147:exon:1000;Parent=CLUHART00000006147 | ||
scaffold789 maker CDS 558191 560123 . + 0 ID=CLUHART00000006147:cds;Parent=CLUHART00000006147 | ||
scaffold789 maker CDS 561401 561519 . + 2 ID=CLUHART00000006147:cds;Parent=CLUHART00000006147 | ||
scaffold789 maker CDS 562057 562121 . + 0 ID=CLUHART00000006147:cds;Parent=CLUHART00000006147 | ||
scaffold789 maker CDS 564372 564588 . + 1 ID=CLUHART00000006147:cds;Parent=CLUHART00000006147 | ||
scaffold789 maker five_prime_UTR 558184 558190 . + . ID=CLUHART00000006147:five_prime_utr;Parent=CLUHART00000006147 | ||
scaffold789 maker three_prime_UTR 564589 564780 . + . ID=CLUHART00000006147:three_prime_utr;Parent=CLUHART00000006147 |
16 changes: 16 additions & 0 deletions
16
src/agat/agat_sp_alignment_output_style/test_data/agat_sp_alignment_output_style_1.gff
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@@ -0,0 +1,16 @@ | ||
##gff-version 3 | ||
scaffold625 maker match 337818 343277 . + . ID=CLUHART00000008717 | ||
scaffold625 maker match_part 337818 337971 . + . ID=CLUHART00000008717:exon:1404;Parent=CLUHART00000008717;Target=CLUHART00000008717 1 154 + | ||
scaffold625 maker match_part 340733 340841 . + . ID=CLUHART00000008717:exon:1405;Parent=CLUHART00000008717;Target=CLUHART00000008717 155 263 + | ||
scaffold625 maker match_part 341518 341628 . + . ID=CLUHART00000008717:exon:1406;Parent=CLUHART00000008717;Target=CLUHART00000008717 264 374 + | ||
scaffold625 maker match_part 341964 343277 . + . ID=CLUHART00000008717:exon:1407;Parent=CLUHART00000008717;Target=CLUHART00000008717 375 1688 + | ||
scaffold789 maker match 558184 564780 . + . ID=CLUHART00000006146 | ||
scaffold789 maker match_part 558184 560123 . + . ID=CLUHART00000006146:exon:995;Parent=CLUHART00000006146;Target=CLUHART00000006146 1 1940 + | ||
scaffold789 maker match_part 561401 561519 . + . ID=CLUHART00000006146:exon:996;Parent=CLUHART00000006146;Target=CLUHART00000006146 1941 2059 + | ||
scaffold789 maker match_part 564171 564235 . + . ID=CLUHART00000006146:exon:997;Parent=CLUHART00000006146;Target=CLUHART00000006146 2060 2124 + | ||
scaffold789 maker match_part 564372 564780 . + . ID=CLUHART00000006146:exon:998;Parent=CLUHART00000006146;Target=CLUHART00000006146 2125 2533 + | ||
scaffold789 maker match 558184 564780 . + . ID=CLUHART00000006147 | ||
scaffold789 maker match_part 558184 560123 . + . ID=CLUHART00000006147:exon:997;Parent=CLUHART00000006147;Target=CLUHART00000006147 1 1940 + | ||
scaffold789 maker match_part 561401 561519 . + . ID=CLUHART00000006147:exon:998;Parent=CLUHART00000006147;Target=CLUHART00000006147 1941 2059 + | ||
scaffold789 maker match_part 562057 562121 . + . ID=CLUHART00000006147:exon:999;Parent=CLUHART00000006147;Target=CLUHART00000006147 2060 2124 + | ||
scaffold789 maker match_part 564372 564780 . + . ID=CLUHART00000006147:exon:1000;Parent=CLUHART00000006147;Target=CLUHART00000006147 2125 2533 + |
10 changes: 10 additions & 0 deletions
10
src/agat/agat_sp_alignment_output_style/test_data/script.sh
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@@ -0,0 +1,10 @@ | ||
#!/bin/bash | ||
|
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# clone repo | ||
if [ ! -d /tmp/agat_source ]; then | ||
git clone --depth 1 --single-branch --branch master https://github.com/NBISweden/AGAT /tmp/agat_source | ||
fi | ||
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# copy test data | ||
cp -r /tmp/agat_source/t/scripts_output/in/0.gff src/agat/agat_sp_alignment_output_style/test_data | ||
cp -r /tmp/agat_source/t/scripts_output/out/agat_sp_alignment_output_style_1.gff src/agat/agat_sp_alignment_output_style/test_data |
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